AB273276

Aldose Reductase Activity Kit (Colorimetric)

Name: Aldose Reductase Activity Kit (Colorimetric) (ab273276) | Abcam
Brand: Abcam Limited
SKU: AB273276
Price: 95000 JPY
Availability: InStock

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(3 Publications)

Aldose Reductase Activity Assay Kit (Colorimetric) (ab273276) uses the ability of Aldose Reductase (AR) to catalyze the oxidation of NADPH.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

別名を表示する

ALDR1, ALR2, AKR1B1, Aldo-keto reductase family 1 member B1, Aldehyde reductase, Aldose reductase, AR

3 Images

Functional Studies - Aldose Reductase Activity Kit (Colorimetric) (AB273276)

FuncS

Supplier Data

Functional Studies - Aldose Reductase Activity Kit (Colorimetric) (AB273276)

Purified Aldose Reductase activity.

FuncS

Supplier Data

Functional Studies - Aldose Reductase Activity Kit (Colorimetric) (AB273276)

NADPH standard curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

FuncS

Supplier Data

Functional Studies - Aldose Reductase Activity Kit (Colorimetric) (AB273276)

Aldose Reductase Activity in Tissue Samples.

Aldose Reductase specific activity was calculated in Rat Kidney tissue lysates (20 μg protein) and HepG2 cell lysates (110 μg protein).

Key facts

検出方法

Colorimetric

サンプルタイプ

Tissue, Suspension cells, Adherent cells

アッセイタイプ

Enzyme activity (quantitative)

検出感度

= 100 µU

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Aldose Reductase Activity Assay Kit (Colorimetric) (ab273276) utilizes the ability of Aldose Reductase (AR) to catalyze the oxidation of NADPH. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm. The assay has been optimized to be monitored using a 96 well plate. The kit can detect as low as 0.1 mU. The assay kit is simple and can be used in a high-throughput format.

This product is manufactured by BioVision, an Abcam company and was previously called K369 Aldose Reductase Activity Kit (Colorimetric). K369-100 is the same size as the 100 test size of ab273276.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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製品内容

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273276-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Active Aldose Reductase", "number":"AB273276-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well UV Transparent Plate", "number":"AB273276-CMP06", "productcode":"" }, { "size":"1 x 35 mL", "name":"AR Assay Buffer", "number":"AB273276-CMP02", "productcode":"" }, { "size":"1 x 400 µL", "name":"DTT I (1M)", "number":"AB273276-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADPH V", "number":"AB273276-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"AR Substrate", "number":"AB273276-CMP03", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

Please refer to protocols

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Aldose reductase also known as AR or ALR2 is an important enzyme in the polyol pathway. It has a molecular mass of about 36 kDa and reduces aldehydes including glucose to their corresponding alcohols. Aldose reductase is expressed widely in various tissues such as kidney lens and retina. The enzyme uses NADPH as a cofactor which is important for its catalytic activity.

Biological function summary

The main function of aldose reductase involves converting glucose to sorbitol a process particularly active under hyperglycemic conditions. It does not form a part of a larger complex but works individually to facilitate this conversion. Sorbitol the product of its enzymatic reaction eventually converts to fructose through the action of sorbitol dehydrogenase. This two-step conversion can contribute to cellular damage due to osmotic stress when sorbitol accumulates.

Pathways

Aldose reductase plays a central role in the polyol pathway. This pathway becomes highly relevant when blood glucose levels are elevated. During such conditions aldose reductase activity increases to convert excess glucose to sorbitol. This activity links it to other proteins like sorbitol dehydrogenase which completes the conversion to fructose.

Aldose reductase has strong connections to diabetic complications specifically diabetic neuropathy and retinopathy. The enzyme’s increased activity under high glucose conditions can lead to osmotic stress and tissue damage. Interactions with other proteins in the metabolic shift associated with diabetes such as sorbitol dehydrogenase underline its involvement. Aldose reductase inhibitors show potential in mitigating these adverse effects by reducing sorbitol accumulation.

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製品プロトコール

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ターゲットの情報

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia (PubMed : 1936586). Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed : 12732097, PubMed : 19010934, PubMed : 8343525). Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed : 17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed : 21329684).

See full target information AKR1B1

追加のターゲット

AKR1B10,AKR1A1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Chemico-biological interactions 392:110905 PubMed38373627

2024

High incidence of sebaceous gland inflammation in aldose reductase-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Anjali Mandava,Binhan Pham,Michelle Pedler,Biehuoy Shieh,Hari Gopalakrishnan,J Mark Petrash

Cell reports. Medicine 4:101056 PubMed37257447

2023

Tracer metabolomics reveals the role of aldose reductase in glycosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Radenkovic,Anna N Ligezka,Sneha S Mokashi,Karen Driesen,Lynn Dukes-Rimsky,Graeme Preston,Luckio F Owuocha,Leila Sabbagh,Jehan Mousa,Christina Lam,Andrew Edmondson,Austin Larson,Matthew Schultz,Pieter Vermeersch,David Cassiman,Peter Witters,Lesa J Beamer,Tamas Kozicz,Heather Flanagan-Steet,Bart Ghesquière,Eva Morava

Clinical and experimental pharmacology & physiology 49:264-274 PubMed34570396

2021

Propofol suppresses cell proliferation in gastric cancer cells through NRF2-mediated polyol pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Cao,Long Fan,Linkai Li,Jiexian Zhou

View all publications

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Aldose Reductase Activity Kit (Colorimetric)