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Acid Phosphatase Assay Kit (Colorimetric) (ab83367)

Submit a review Q&A (2)References (14)
Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
  • Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 1 hr 10 min
  • Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
  • Sensitivity: 0.001 mM

製品の概要

  • 製品名

    Acid Phosphatase Assay Kit (Colorimetric)
    Acid phosphatase キット 製品一覧

  • 検出方法

    Colorimetric

  • サンプルの種類

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media

  • アッセイタイプ

    Enzyme activity

  • 検出感度

    > 0.001 mM

  • 検出範囲

    0.001 mM - 1 mM

  • 全工程の試験時間

    1h 10m

  • 製品の概要

    Acid Phosphatase Assay Kit ab83367 is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure acid phosphatase activity in serum and other samples.


    The acid phosphatase assay protocol uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (absorbance max 405 nm) when dephosphorylated by acid phosphatase.


    Acid phosphatase assay protocol summary:
    - add samples and standards to wells
    - add pNPP to samples
    - add acid phosphatase enzyme to standards
    - incubate for 60 min
    - add stop solution
    - analyze with microplate reader

  • 特記事項

    This product is manufactured by BioVision, an Abcam company and was previously called K411 Acid Phosphatase Activity Colorimetric Assay Kit. K411-500 is the same size as the 500 test size of ab83367.

    Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease.

    pNPP Assays

    pNPP assays are a class of phosphatase assays using p-nitrophenyl phosphate (pNPP) as a phosphatase substrate. They can be used to measure alkaline phosphatase, neutral phosphatase, and acid phosphatase activity. These different types of phosphatases are active respectively in alkaline, neutral, and acid assay buffers. pNPP assays include Alkaline Phosphatase Assay Kit ab83369 and Acid Phosphatase Assay Kit ab83367.

  • 試験プラットフォーム

    Microplate reader

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 500 tests
    AP Assay Buffer 1 x 100ml
    AP Enzyme 1 vial
    pNPP Substrate 1 vial
    Stop Solution I 1 x 10ml

  • 研究分野

  • 関連性

    Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.

  • 細胞内局在

    ACP1: Cytoplasm. ACP2: Lysosome membrane; Single-pass membrane protein. ACP5: Lysosome. ACPP: Isoform 1: Secreted. Isoform 2: Lysosome membrane; Single-pass type I membrane protein.

  • 別名

    • Acid phosphatase 1 soluble
    • Acid phosphatase 2 lysosomal
    • Acid phosphatase 5 tartrate resistant
    • Acid phosphatase of erythrocyte
    • Acid phosphatase prostate
    • ACP1
    • ACP2
    • ACP3
    • ACP5
    • ACPP
    • Adipocyte acid phosphatase
    • HAAP
    • LAP
    • LMW PTP
    • LMW PTPase
    • Low molecular weight cytosolic acid phosphatase
    • Low molecular weight phosphotyrosine protein phosphatase
    • Lysosomal acid phosphatase
    • PAP
    • pnpp
    • Prostatic acid phosphotase
    • Red cell acid phosphatase 1
    • Tartrate resistant acid ATPase
    • Tartrate resistant acid phosphatase 5
    • Tartrate resistant acid phosphatase type 5
    • TR AP
    • TRAP
    • TrATPase
    see all

画像

  • Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
    Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
    AP Enzyme samples 40 minute assay
  • Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
    Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
    p-Nitrophenol (pNP) standard curve

参考文献 (14)

ab83367 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab83367 は 14 報の論文で使用されています。

  • Pastwinska J  et al. Hypoxia modulates human mast cell adhesion to hyaluronic acid. Immunol Res 70:152-160 (2022). PubMed: 34791576
  • Zheng ZG  et al. Discovery of a potent SCAP degrader that ameliorates HFD-induced obesity, hyperlipidemia and insulin resistance via an autophagy-independent lysosomal pathway. Autophagy 17:1592-1613 (2021). PubMed: 32432943
  • Yang H  et al. NF2 and Canonical Hippo-YAP Pathway Define Distinct Tumor Subsets Characterized by Different Immune Deficiency and Treatment Implications in Human Pleural Mesothelioma. Cancers (Basel) 13:N/A (2021). PubMed: 33805359
  • Yang H  et al. Pharmaco-transcriptomic correlation analysis reveals novel responsive signatures to HDAC inhibitors and identifies Dasatinib as a synergistic interactor in small-cell lung cancer. EBioMedicine 69:103457 (2021). PubMed: 34224975
  • Pastwinska J  et al. Hypoxia regulates human mast cell adhesion to fibronectin via the PI3K/AKT signaling pathway. Cell Adh Migr 14:106-117 (2020). PubMed: 32427041
View all Publications for this product

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Question

I am enquiring as to whether this kit that can be used as a tartrate-resistant acid phosphatase quantification assay. The nature of my samples are hard to visualise microscopically so a TRAP stain will not work. I haven't kept the supernatant either so TRAP quantification in the media is not possible. Can this assay be used to quantify the amount of TRAP in the cell lysate, do you think this would work?

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Answer



If the TRAP is able to perform the same functions (phosphorylation of the substrates) as any other AP, then yes, this kit could be used. However, we cannot guarantee that it will definitely work as we have never tried it to quantify TRAP.

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Question

Could you please provide the buffer constituents details with information on molar, pH, presence/absence of protease inhibitor, etc?

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Answer

Thank you for your enquiry.

Unfortunately, most of the information you asked for is proprietary info. However, I can confirm that the assay buffer does not contain protease inhibitors and its pH is 5.0.

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