AB83367

Acid Phosphatase Assay Kit (Colorimetric)

JP Deleterious:医薬用外劇物

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(15 Publications)

Acid Phosphatase Assay Kit ab83367 is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure acid phosphatase activity in serum and other samples.

別名を表示する

Lysosomal acid phosphatase, LAP, ACP2

2 Images

Functional Studies - Acid Phosphatase Assay Kit (Colorimetric) (AB83367)

FuncS

Supplier Data

Functional Studies - Acid Phosphatase Assay Kit (Colorimetric) (AB83367)

p-Nitrophenol (pNP) standard curve

FuncS

Supplier Data

Functional Studies - Acid Phosphatase Assay Kit (Colorimetric) (AB83367)

AP Enzyme samples 40 minute assay

Key facts

検出方法

Colorimetric

サンプルタイプ

Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate

アッセイタイプ

Enzyme activity

検出感度

> 0.001 mM

検出範囲

0.001 - 1 mM

アッセイ時間

1h 10m

アッセイプラットフォーム

Microplate reader

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Reactivity data

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製品の詳細

Acid Phosphatase Assay Kit ab83367 is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure acid phosphatase activity in serum and other samples.

The acid phosphatase assay protocol uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (absorbance max 405 nm) when dephosphorylated by acid phosphatase.

Acid phosphatase assay protocol summary:
- add samples and standards to wells
- add pNPP to samples
- add acid phosphatase enzyme to standards
- incubate for 60 min
- add stop solution
- analyze with microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K411 Acid Phosphatase Activity Colorimetric Assay Kit. K411-500 is the same size as the 500 test size of ab83367.

Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease.

pNPP Assays

pNPP assays are a class of phosphatase assays using p-nitrophenyl phosphate (pNPP) as a phosphatase substrate. They can be used to measure alkaline phosphatase, neutral phosphatase, and acid phosphatase activity. These different types of phosphatases are active respectively in alkaline, neutral, and acid assay buffers. pNPP assays include Alkaline Phosphatase Assay Kit ab83369 and Acid Phosphatase Assay Kit ab83367.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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製品内容

{ "values": { "500Test": { "sellingSize": "500 Test", "publicAssetCode":"ab83367-500Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"AP Enzyme", "number":"AB83367-CMP02", "productcode":"" }, { "size":"1 x 100 mL", "name":"AP Assay Buffer", "number":"AB83367-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"pNPP Substrate", "number":"AB83367-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution I", "number":"AB83367-CMP04", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Acid phosphatase also known as tartrate-resistant acid phosphatase (TRAP) or prostatic acid phosphatase (PAP) is an enzyme that catalyzes the hydrolysis of phosphate esters in an acidic environment. It has a molecular mass of approximately 50 kDa and is expressed in various human tissues including the prostate bone liver and spleen. Within the prostate it breaks down and releases phosphate from different compounds and it plays a role in bone resorption by osteoclasts.

Biological function summary

This enzyme contributes to multiple cellular processes including the regulation of bone metabolism and prostate health. Acid phosphatase when acting as TRAP is often found within osteoclast lysosomes where it degrades bone matrix components. It does not form part of a complex but functions independently to modulate these processes. Prostatic acid phosphatase in seminal fluid has additional roles particularly in prostate tissue affecting cellular proliferation and motility.

Pathways

Acid phosphatase participates in both bone resorption and lysosomal degradation pathways. In bone metabolism it works alongside proteins such as cathepsin K assisting in the breakdown of matrix constituents during bone remodeling. In lysosomal pathways it complements activities of enzymes like lysosomal acid lipase helping in recycling cellular materials. These interactions underline its involvement in maintaining cellular homeostasis through these critical biological pathways.

Acid phosphatase relates significantly to prostate cancer and bone disorders like osteoporosis. Elevated levels of prostatic acid phosphatase in serum serve as a marker for prostate cancer linking it to androgen receptor pathways critical in disease progression. In bone disorders TRAP's role in osteoclast function connects it to conditions such as osteoporosis where its interaction with integrin receptor influences bone density regulation. Understanding these connections can aid in developing diagnostic and therapeutic strategies for these diseases.

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製品プロトコール

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ターゲットの情報

See full target information ACP2

追加のターゲット

Tartrate-resistant acid phosphatase type 5,ACP3,ACP1

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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 9:55 PubMed36765038

2023

MEK1 drives oncogenic signaling and interacts with PARP1 for genomic and metabolic homeostasis in malignant pleural mesothelioma.

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Haitang Yang,Yanyun Gao,Duo Xu,Ke Xu,Shun-Qing Liang,Zhang Yang,Amina Scherz,Sean R R Hall,Stefan Forster,Sabina Berezowska,Feng Yao,Adrian F Ochsenbein,Thomas M Marti,Gregor J Kocher,Ralph A Schmid,Patrick Dorn,Ren-Wang Peng

Immunologic research 70:152-160 PubMed34791576

2021

Hypoxia modulates human mast cell adhesion to hyaluronic acid.

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Joanna Pastwińska,Aurelia Walczak-Drzewiecka,Elżbieta Kozłowska,Enjuro Harunari,Marcin Ratajewski,Jarosław Dastych

EBioMedicine 69:103457 PubMed34224975

2021

Pharmaco-transcriptomic correlation analysis reveals novel responsive signatures to HDAC inhibitors and identifies Dasatinib as a synergistic interactor in small-cell lung cancer.

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Haitang Yang,Beibei Sun,Ke Xu,Yunfei He,Tuo Zhang,Sean R R Hall,Swee T Tan,Ralph A Schmid,Ren-Wang Peng,Guohong Hu,Feng Yao

Cancers 13: PubMed33805359

2021

NF2 and Canonical Hippo-YAP Pathway Define Distinct Tumor Subsets Characterized by Different Immune Deficiency and Treatment Implications in Human Pleural Mesothelioma.

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Unspecified application

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Haitang Yang,Sean R R Hall,Beibei Sun,Liang Zhao,Yanyun Gao,Ralph A Schmid,Swee T Tan,Ren-Wang Peng,Feng Yao

Cancers 12: PubMed32824422

2020

Systematic Analysis of Aberrant Biochemical Networks and Potential Drug Vulnerabilities Induced by Tumor Suppressor Loss in Malignant Pleural Mesothelioma.

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Unspecified application

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Haitang Yang,Duo Xu,Zhang Yang,Feng Yao,Heng Zhao,Ralph A Schmid,Ren-Wang Peng

Autophagy 17:1592-1613 PubMed32432943

2020

Discovery of a potent SCAP degrader that ameliorates HFD-induced obesity, hyperlipidemia and insulin resistance via an autophagy-independent lysosomal pathway.

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Zu-Guo Zheng,Si-Tong Zhu,Hui-Min Cheng,Xin Zhang,Gang Cheng,Pyone Myat Thu,Supeng Perry Wang,Hui-Jun Li,Ming Ding,Lei Qiang,Xiao-Wei Chen,Qing Zhong,Ping Li,Xiaojun Xu

Cell adhesion & migration 14:106-117 PubMed32427041

2020

Hypoxia regulates human mast cell adhesion to fibronectin via the PI3K/AKT signaling pathway.

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Unspecified application

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Unspecified reactive species

Joanna Pastwińska,Aurelia Walczak-Drzewiecka,Magdalena Łukasiak,Marcin Ratajewski,Jarosław Dastych

Oncogenesis 8:45 PubMed31431614

2019

HSP90/AXL/eIF4E-regulated unfolded protein response as an acquired vulnerability in drug-resistant KRAS-mutant lung cancer.

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Unspecified application

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Haitang Yang,Shun-Qing Liang,Duo Xu,Zhang Yang,Thomas M Marti,Yanyun Gao,Gregor J Kocher,Heng Zhao,Ralph A Schmid,Ren-Wang Peng

Scientific reports 9:11635 PubMed31406169

2019

Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe.

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Unspecified application

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Shunsuke Ishii,Akira Matsuura,Eisuke Itakura

Cancer letters 449:252-262 PubMed30790678

2019

Proton pump inhibitors promote the growth of androgen-sensitive prostate cancer cells through ErbB2, ERK1/2, PI3K/Akt, GSK-3β signaling and inhibition of cellular prostatic acid phosphatase.

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Unspecified application

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Unspecified reactive species

Iacopo Gesmundo,Laura Di Blasio,Dana Banfi,Tania Villanova,Alessandro Fanciulli,Enrica Favaro,Giacomo Gamba,Chiara Musuraca,Ida Rapa,Marco Volante,Stefania Munegato,Mauro Papotti,Paolo Gontero,Luca Primo,Ezio Ghigo,Riccarda Granata

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Abcam product promise

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Acid Phosphatase Assay Kit (Colorimetric)