2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) (ab174097)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: Adherent cells, Cell culture extracts, Suspension cells, Tissue Extracts
製品の概要
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製品名
2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Cell culture extracts, Adherent cells, Suspension cells, Tissue Extracts -
アッセイタイプ
Quantitative -
種交差性
交差種: Mammals, Other species -
製品の概要
Abcam’s 2-Phosphoglycerate Assay kit (ab174097) is a sensitive, fast and easy-to-use kit. In this assay, 2PG(2-phosphoglycerate) is converted by Enzyme Mix to PEP (phosphoenolpyruvate), which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The colored product or fluorescence intensity is proportional to 2PG level. This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.
Visit our FAQs page for tips and troubleshooting.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K778 2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit. K778-100 is the same size as the 100 test size of ab174097.
2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2PG is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool for analyzing the glycolytic pathway and its relevance to cancer research.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 2PG Assay Buffer 1 x 25ml 2PG Converter 1 vial 2PG Developer 1 vial 2PG Enzyme Mix (Lyophilized) 1 vial 2PG Probe (in DMSO) 1 x 0.2ml 2PG Standard 1 vial -
研究分野
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関連性
2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2-Phosphoglycerate is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). -
別名
- 2 Phosphoglycerate
- 2PG
画像
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Example of 2PG Standard curve obtained with 2-PG Assay Kit (ab174097) by colorimetric reading. Please not this data is example data only.
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Example of 2PG Standard curve obtained with 2-PG Assay Kit (ab174097) by fluorometric reading. Please not this data is example data only.
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Measurement of 2PG level in rat liver, heart and muscle lysate (200 µg protein each). Assays were performed according to kit protocol. This is example data only.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab174097 は 3 報の論文で使用されています。
- Jiang Y et al. Aurora A-mediated pyruvate kinase M2 phosphorylation promotes biosynthesis with glycolytic metabolites and tumor cell cycle progression. J Biol Chem 298:102561 (2022). PubMed: 36198360
- Song J et al. Dysregulation of the NUDT7-PGAM1 axis is responsible for chondrocyte death during osteoarthritis pathogenesis. Nat Commun 9:3427 (2018). PubMed: 30143643
- Wu C et al. A natural inhibitor of kidney-type glutaminase: a withanolide from Physalis pubescens with potent anti-tumor activity. Oncotarget 8:113516-113530 (2017). PubMed: 29371926