2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) (ab174097)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: Adherent cells, Cell culture extracts, Suspension cells, Tissue Extracts
製品の概要
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製品名
2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Cell culture extracts, Adherent cells, Suspension cells, Tissue Extracts -
アッセイタイプ
Quantitative -
種交差性
交差種: Mammals, Other species -
製品の概要
Abcam’s 2-Phosphoglycerate Assay kit (ab174097) is a sensitive, fast and easy-to-use kit. In this assay, 2PG(2-phosphoglycerate) is converted by Enzyme Mix to PEP (phosphoenolpyruvate), which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The colored product or fluorescence intensity is proportional to 2PG level. This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.
Visit our FAQs page for tips and troubleshooting.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K778 2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit. K778-100 is the same size as the 100 test size of ab174097.
2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2PG is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool for analyzing the glycolytic pathway and its relevance to cancer research.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 2PG Assay Buffer 1 x 25ml 2PG Converter 1 vial 2PG Developer 1 vial 2PG Enzyme Mix (Lyophilized) 1 vial 2PG Probe (in DMSO) 1 x 0.2ml 2PG Standard 1 vial -
研究分野
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関連性
2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2-Phosphoglycerate is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). -
別名
- 2 Phosphoglycerate
- 2PG
画像
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2PG Standard Curve (Colorimetric)
Example of 2PG Standard curve obtained with 2-PG Assay Kit (ab174097) by colorimetric reading. Please not this data is example data only.
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2PG Standard Curve (Fluoroimetric)Example of 2PG Standard curve obtained with 2-PG Assay Kit (ab174097) by fluorometric reading. Please not this data is example data only.
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Measurement of 2PG levelsMeasurement of 2PG level in rat liver, heart and muscle lysate (200 µg protein each). Assays were performed according to kit protocol. This is example data only.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab174097 は 3 報の論文で使用されています。
- Jiang Y et al. Aurora A-mediated pyruvate kinase M2 phosphorylation promotes biosynthesis with glycolytic metabolites and tumor cell cycle progression. J Biol Chem 298:102561 (2022). PubMed: 36198360
- Song J et al. Dysregulation of the NUDT7-PGAM1 axis is responsible for chondrocyte death during osteoarthritis pathogenesis. Nat Commun 9:3427 (2018). PubMed: 30143643
- Wu C et al. A natural inhibitor of kidney-type glutaminase: a withanolide from Physalis pubescens with potent anti-tumor activity. Oncotarget 8:113516-113530 (2017). PubMed: 29371926