Mouse Cytokine 抗体 Array - Membrane (97 targets) (ab169820)
製品の概要
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製品名
Mouse Cytokine Antibody Array - Membrane (97 targets)
Cytokine Antibody Array antibody arrays 製品一覧 -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
アッセイタイプ
Semi-quantitative -
種交差性
交差種: Mouse -
製品の概要
Abcam Mouse Cytokine Antibody Array - Membrane (ab169820) detects 97 Mouse Cytokines. Suitable for all liquid sample types.
Targets: 6Ckine, ALK-1, Amphiregulin, Axl, BLC, Cardiotrophin-1, CD27, CD27 Ligand, CD30, CD30 Ligand, CD36/SR-B3, CTLA-4, CXCL16, Decorin, Dkk-1, E-Cadherin, EGF, Eotaxin-1, Eotaxin-2, Epigen, E-Selectin, Fas Ligand, Fc gamma RIIB, Flt-3 Ligand, Fractalkine, Galectin-1, Gas 6, G-CSF, GITR, GITR Ligand, Granzyme B, HAI-1, HGF, IFN-gamma, IGFBP-5, IGFBP-6, IGF-2, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-17, IL-17B R, IL-17E, IL-17F, IL-20, IL-21, I-TAC, JAM-A, KC/CXCL1, Leptin/OB, Leptin R, L-Selectin, Lungkine, MadCAM-1, MCP-1, MDC, MFG-E8, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, MIP-3 beta, MMP-2, Osteopontin, Osteoprotegerin, Prolactin, Pro-MMP-9, RANTES, SCF, sTNFRI, sTNFRII, TACI, TARC, TNF alpha, Thrombopoietin, TRANCE, TROY, TWEAK R, VCAM-1, VEGF-A, VEGFR1, VEGFR3, VEGF-D
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about membrane antibody arrays
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特記事項
If you are interested in this cytokine array, a table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Multiplex Protein Detectionmore details
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 2 x 50µl 1 x 50µl 1X Blocking Buffer 2 x 25ml 4 x 25ml 1 x 25ml 20X Wash Buffer I 1 x 20ml 2 x 20ml 2 x 10ml 20X Wash Buffer II 1 x 20ml 2 x 20ml 1 x 10ml 2X Cell Lysis Buffer 1 x 16ml 2 x 16ml 1 x 10ml 4000X Biotin-Conjugated Anti Cytokines 4 vials 8 vials 2 vials 4-Well Plastic Tray 1 unit 2 units 1 unit Cytokine Antibody Array Membranes 4 units 8 units 2 units Detection Buffer C 1 x 2.5ml 2 x 2.5ml 1 x 1.5ml Detection Buffer D 1 x 2.5ml 2 x 2.5ml 1 x 1.5ml -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab169820の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Multiplex Protein Detection |
Use at an assay dependent concentration.
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| 特記事項 |
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Multiplex Protein Detection
Use at an assay dependent concentration. |
画像
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Multiplex Protein Detection - Mouse Cytokine Antibody Array - Membrane (97 targets) (ab169820)Typical results obtained with Abcam Membrane Antibody Arrays. The membrane was probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab169820 は 3 報の論文で使用されています。
- Wuestefeld A et al. A Pro-Regenerative Environment Triggers Premalignant to Malignant Transformation of Senescent Hepatocytes. Cancer Res 83:428-440 (2023). PubMed: 36449018
- Sodir NM et al. MYC Instructs and Maintains Pancreatic Adenocarcinoma Phenotype. Cancer Discov 10:588-607 (2020). PubMed: 31941709
- Hartman ES et al. Increased Reactive Oxygen Species and Cell Cycle Defects Contribute to Anemia in the RASA3 Mutant Mouse Model scat. Front Physiol 9:689 (2018). PubMed: 29922180