AB197421

Human Angiogenesis 抗体 Array B (30 Targets)- Quantitative

Name: Human Angiogenesis 抗体 Array B (30 Targets)- Quantitative (ab197421)
Brand: Abcam Limited
SKU: AB197421
Price: 249000 JPY
Availability: InStock

Human Angiogenesis Antibody Array B (30 Targets)- Quantitative

Be the first to review this product! Submit a review

(0 Publication)

ab197421 can be used for quantitative measurement of 30 Human Angiogenesis proteins.

別名を表示する

RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5

2 Images

Multiplex Protein Detection - Human Angiogenesis Antibody Array B (30 Targets)- Quantitative (AB197421)

Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Angiogenesis Antibody Array B (30 Targets)- Quantitative (AB197421)

Array Map for Abcam Human Angiogenesis Antibody Array B (30 Targets) - Quantitative (ab197421)

Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Angiogenesis Antibody Array B (30 Targets)- Quantitative (AB197421)

Standard Curves obtained from Abcam Human Angiogenesis Antibody Array B (30 Targets) - Quantitative (ab197421)

Key facts

アッセイタイプ

Quantitative

交差種

Human

ターゲット

ANG

target

検出方法

Fluorescent

アッセイプラットフォーム

Multiplex

サンプルタイプ

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

style

left-column

製品の詳細

ab197421 can be used for quantitative measurement of 30 Human Angiogenesis proteins. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Targets: ANG-1, Angiostatin, CXCL16, EGF, FGF-4, Follistatin, G-CSF, GM-CSF, I-309, IL-1β, IL-4, IL-10, IL-12p40, IL-12p70, I-TAC, MCP-2, MCP-3, MCP-4, MMP-1, MMP-9, PECAM-1, TGFα, TGFβ3, Tie-1, Tie-2, uPAR, VEGF, VEGF R2, VEGF R3, VEGF-D.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. The standard is affinity purified. "

style

left-column

製品内容

{ "values": { "8Test": { "sellingSize": "8 Test", "publicAssetCode":"ab197421-8Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Adhesive Film", "number":"AB197421-CMP09" }, { "size":"1 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197421-CMP02" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197421-CMP07" }, { "size":"1 x 15 mL", "name":"Sample Diluent", "number":"AB197421-CMP01" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197421-CMP06" }, { "size":"1 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197421-CMP08" }, { "size":"1 x 25 µL", "name":"Human Angiogenesis Array B (30 T) Biotinylated Antibody Cocktail", "number":"AB197421-CMP03" }, { "size":"1 x 1 Vial", "name":"Human Angiogenesis Array B (30 T) Lyophilized Cytokine Standard Mix", "number":"AB197421-CMP05" }, { "size":"1 x 1 Slide", "name":"Human Angiogenesis Array B (30 T) Glass Slide", "number":"AB197421-CMP04" } ] }, "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab197421-50Test", "assetComponentDetails": [ { "size":"4 x 1 Unit", "name":"Adhesive Film", "number":"AB197421-CMP09" }, { "size":"2 x 1 Vial", "name":"Human Angiogenesis Array B (30 T) Lyophilized Cytokine Standard Mix", "number":"AB197421-CMP05" }, { "size":"6 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197421-CMP06" }, { "size":"2 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197421-CMP02" }, { "size":"2 x 15 mL", "name":"Sample Diluent", "number":"AB197421-CMP01" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197421-CMP07" }, { "size":"4 x 25 µL", "name":"Human Angiogenesis Array B (30 T) Biotinylated Antibody Cocktail", "number":"AB197421-CMP03" }, { "size":"4 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197421-CMP08" }, { "size":"4 x 1 Slide", "name":"Human Angiogenesis Array B (30 T) Glass Slide", "number":"AB197421-CMP04" } ] }, "22Test": { "sellingSize": "22 Test", "publicAssetCode":"ab197421-22Test", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"Adhesive Film", "number":"AB197421-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197421-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"Sample Diluent", "number":"AB197421-CMP01", "productcode":"" }, { "size":"2 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197421-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human Angiogenesis Array B (30 T) Lyophilized Cytokine Standard Mix", "number":"AB197421-CMP05", "productcode":"" }, { "size":"2 x 25 µL", "name":"Human Angiogenesis Array B (30 T) Biotinylated Antibody Cocktail", "number":"AB197421-CMP03", "productcode":"" }, { "size":"2 x 1 Slide", "name":"Human Angiogenesis Array B (30 T) Glass Slide", "number":"AB197421-CMP04", "productcode":"" }, { "size":"3 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197421-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197421-CMP02", "productcode":"" } ] } } }

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

style

left-column

style

left-column

製品プロトコール

style

left-column

ターゲットの情報

Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context (PubMed : 12051708, PubMed : 1400510, PubMed : 19332886, PubMed : 20129916, PubMed : 21855800, PubMed : 23047679, PubMed : 23843625, PubMed : 2424496, PubMed : 2459697, PubMed : 2730651, PubMed : 27518564, PubMed : 28176817, PubMed : 29100074, PubMed : 29748193, PubMed : 3122207, PubMed : 32510170, PubMed : 38718836, PubMed : 8159680, PubMed : 8570639, PubMed : 8622921, PubMed : 9578571). Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus (PubMed : 29100074, PubMed : 32510170). Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs) : specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation (PubMed : 1400510, PubMed : 19332886, PubMed : 20129916, PubMed : 21855800, PubMed : 23047679, PubMed : 27518564, PubMed : 29100074, PubMed : 29748193, PubMed : 32510170, PubMed : 38718836). tiRNas also prevent formation of apoptosome, thereby promoting cell survival (By similarity). Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36 (PubMed : 3289612, PubMed : 38718836). Cleaves a subset of tRNAs, including tRNA(Ala), tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), tRNA(Asp) and tRNA(Sec) (PubMed : 31582561). Under growth conditions and in differentiated cells, translocates to the nucleus and stimulates ribosomal RNA (rRNA) transcription, including that containing the initiation site sequences of 45S rRNA, thereby promoting cell growth and proliferation (PubMed : 12051708, PubMed : 15735021, PubMed : 27518564, PubMed : 29100074, PubMed : 8127865). Angiogenin induces vascularization of normal and malignant tissues via its ability to promote rRNA transcription (PubMed : 19354288, PubMed : 4074709, PubMed : 8448182). Involved in hematopoietic stem and progenitor cell (HSPC) growth and survival by promoting rRNA transcription in growth conditions and inhibiting translation in response to stress, respectively (PubMed : 27518564). Mediates the crosstalk between myeloid and intestinal epithelial cells to protect the intestinal epithelial barrier integrity : secreted by myeloid cells and promotes intestinal epithelial cells proliferation and survival (PubMed : 32510170). Also mediates osteoclast-endothelial cell crosstalk in growing bone : produced by osteoclasts and protects the neighboring vascular cells against senescence by promoting rRNA transcription (By similarity).

See full target information ANG

追加のターゲット

PLG,CXCL16,EGF,FGF4,FST,CSF3,CSF2,CCL1,IL1B,IL10,IL12B,IL12A,IL4,CXCL11,CCL8,CCL7,CCL13,MMP1,PECAM1,Protransforming growth factor alpha,TGFB3,TIE1,TEK,PLAUR,VEGFA,VEGFD,KDR,FLT4

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Angiogenesis 抗体 Array B (30 Targets)- Quantitative

Human Angiogenesis Antibody Array B (30 Targets)- Quantitative

Multiplex Protein Detection - Human Angiogenesis Antibody Array B (30 Targets)- Quantitative (AB197421)

Multiplex Protein Detection - Human Angiogenesis Antibody Array B (30 Targets)- Quantitative (AB197421)

Key facts

アッセイタイプ

交差種

ターゲット

検出方法

アッセイプラットフォーム

サンプルタイプ

製品の詳細

製品内容

出荷温度及び保存条件

出荷温度

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

追加のターゲット

組合せ製品

Multiplex Kits

Multiplex Kits

Multiplex Kits

Multiplex Kits

Abcam product promise