Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-procollagen type IIA 抗体 [2] (ab17771)

Reviews (2)Q&A (4)References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (ab17771)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (ab17771)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (ab17771)

Key features and details

  • Mouse monoclonal [2] to procollagen type IIA
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-procollagen type IIA antibody [2]

  • 製品の詳細

    Mouse monoclonal [2] to procollagen type IIA

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • エピトープ

    Epitope is located at the amino-terminal part of procollagen type IIA.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab17771の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
特記事項
IHC-P
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 関連性

    Collagen type II is the most abundant protein of cartilage matrix. Two isoforms exist and collagen type IIA is involved in early development of cartilage in embryos. The collagen is produced from type II procollagen when two teloterminal propeptides are digested from the precursor and released into the circulation. Procollagen type II N-terminal propeptide is considered a marker of cartilage synthesis and the normal concentration in serum is approximately 30ng/mL.

  • 細胞内局在

    Secreted

  • 参照データベース

    • 別名

      • Alpha 1 type II collagen antibody
      • Collagen II alpha 1 polypeptide antibody
      • Procollagen type II alpha 1 antibody

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (ab17771)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (ab17771)
      ab17771, at a 1/1000 dilution, staining fetal bone anlage in the hand (around week 10) by IHC-P.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (ab17771)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (ab17771)
      ab17771, at a 1/1000 dilution, staining specific tumour cells of biopsy from a chondroblastoma by IHC-P.  The antibody was detected with an HRP conjugated Goat anti-Mouse antibody.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (ab17771)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-procollagen type IIA antibody [2] (ab17771)This image is courtesy of an anonymous Abreview

      ab17771 staining mouse eye tissue sections -- conjunctiva and limbus by IHC-P.  The tissue was fixed with paraformaldehyde and antigen retrieval was heat mediated using citrate buffer.  The blocking agent was 1% TSA at 25°C for 1 hour.  The primary antibody was incubated (undiluted) at 25°C for 15 hours.  A biotinylated anti-mouse was used as the secondary antibody.

      See Abreview

    参考文献 (3)

    ab17771 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab17771 は 3 報の論文で使用されています。

    • Chen L  et al. Overexpression of long non-coding RNA AP001505.9 inhibits human hyaline chondrocyte dedifferentiation. Aging (Albany NY) 13:11433-11454 (2021). PubMed: 33839696
    • Olderøy MØ  et al. Biochemical and structural characterization of neocartilage formed by mesenchymal stem cells in alginate hydrogels. PLoS One 9:e91662 (2014). IHC-P ; Human . PubMed: 24626259
    • Lee CH  et al. Protein-releasing polymeric scaffolds induce fibrochondrocytic differentiation of endogenous cells for knee meniscus regeneration in sheep. Sci Transl Med 6:266ra171 (2014). PubMed: 25504882

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-procollagen type IIA antibody [003-02]

     Inconclusive
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - other (human fibroblasts, chondrocytes,iPSC)
    Loading amount
    30 µg
    Specification
    human fibroblasts, chondrocytes,iPSC
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 21 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-procollagen type IIA antibody [003-02]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (eye--conjunctiva and limbus)
    Specification
    eye--conjunctiva and limbus
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 10 2007

    Question

    Would be interested in trying this antibody in Western blot.

    Read More
    Answer

    Thank you very much for your call today and for your interest in ab17771.

    To our knowledge, ab17771 has not been tested in Western blot. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

    Simply follow these easy steps below to apply for our AbTrial Program:

    1. Reply to this email, letting us know you are interested in testing this product.

    2. We will email you an inactive personal discount code for the value of the product.

    3. Purchase and test the product at the regular price.

    4. Submit your results, including your discount code in the additional notes section of your Abreview.

    5. Once the Abreview is submitted, the discount code will become active.

    6. Apply your discount code on your next order to receive that value off.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

    Read More

    Question

    Will this product work on dog?

    Read More
    Answer

    Thank you for contacting us. After performing a BLAST search on the immunogen sequence, I am pleased to report that there is 97% identity with dog.  Though we cannot guarantee the antibody to work on this species because we do not have any data showing staining in dog tissues, we would expect this to react with dog. If you do choose to try this antibody on dog, please consider submitting an Abreview with your results so that we may share this information with other customers. Please do not hesitate to contact us if you need any further assistance.  Best of luck in your research!

    Read More

    Question

    Please refer to the following query from a customer who is looking for collagen antibodies which recognises the C-propeptide: Thank you for forwarding that information regarding ab17771. Unfortunately the immunogen was the N-propepide and I need an antibody that recognises the C-propeptide and is shown to recognise the mouse protein on Western blots. Could you please help out with this enquiry? Thank you for your time and I look forward to your reply

    Read More
    Answer

    Thank you for your enquiry. Unfortunately ab17771 is the only procollagen type IIA antibody that we currently carry. If you have any additional questions, please contact us again.

    Read More

    Question

    I am interested in your procollagen antibodies. For example, for ab17771, how likely is it that it will cross-react with rat? Do you have any procollagen antibody that you think will cross-react with rat?

    Read More
    Answer

    We currently have only ab17771 which recognises procollagen type IIA and cross reacts with human. A blast search revealed that there is 92% identity between the human and the rat protein (3.5% are conservative substitutions and 3.5% are insertions or substitutions). This antibody is a monoclonal therefore it recognises a single epitope and therefore has less chances than a polyclonal to recognise the rat form. Having not tested it in rat we do not know if it will work in rat. I hope this information will help you, please let me know if you need any further information,

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com