Key features and details
- Rabbit polyclonal to PRMT7
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
PRMT7 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to PRMT7
アプリケーション適用あり: WB, IHC-Pmore details
種交差性交差種: Mouse, Rat, Human
Recombinant fragment, corresponding to a region within amino acids 408-665 of Human PRMT7 (Q9NVM4).
- PC3 whole cell lysate; Rat RT2, Hela and Mouse C2C12 xenograft tissues.
特記事項Keep as concentrated solution.
保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab126965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 78 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
機能Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.
関連疾患Defects in PRMT7 are associated with mild intellectual disabilityy, obesity and symmetrical shortening of the digits and posterior metacarpals and metatarsals. The phenotype is a phenocopy of pseudohypoparathyroidism (PHP).
配列類似性Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily.
Contains 2 SAM-dependent MTase PRMT-type domains.
細胞内局在Cytoplasm, cytosol. Nucleus.
- Information by UniProt
- [Myelin basic protein]-arginine N-methyltransferase PRMT7 antibody
- ANM7_HUMAN antibody
- FLJ10640 antibody
Anti-PRMT7 antibody (ab126965) at 1/2000 dilution + PC3 whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 78 kDa
7.5% SDS PAGE
ab126965, at 1/500 dilution, staining PRMT7 in Paraffin-embedded Rat RT2 xenograft tissue by Immunohistochemistry.
ab126965, at 1/500 dilution, staining PRMT7 in Paraffin-embedded Human Hela xenograft tissue by Immunohistochemistry.
ab126965, at 1/500 dilution, staining PRMT7 in Paraffin-embedded Mouse C2C12 xenograft tissue by Immunohistochemistry.
ab126965 は 1 報の論文で使用されています。
- Gurung B et al. Menin epigenetically represses Hedgehog signaling in MEN1 tumor syndrome. Cancer Res 73:2650-8 (2013). WB . PubMed: 23580576