Anti-PRMT7 抗体 (ab126965)
Key features and details
- Rabbit polyclonal to PRMT7
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PRMT7 antibody
PRMT7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PRMT7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 408-665 of Human PRMT7 (Q9NVM4).
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ポジティブ・コントロール
- PC3 whole cell lysate; Rat RT2, Hela and Mouse C2C12 xenograft tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab126965の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 78 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 78 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo. -
関連疾患
Defects in PRMT7 are associated with mild intellectual disabilityy, obesity and symmetrical shortening of the digits and posterior metacarpals and metatarsals. The phenotype is a phenocopy of pseudohypoparathyroidism (PHP). -
配列類似性
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily.
Contains 2 SAM-dependent MTase PRMT-type domains. -
細胞内局在
Cytoplasm, cytosol. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 54496 Human
- Entrez Gene: 214572 Mouse
- Entrez Gene: 361402 Rat
- Omim: 610087 Human
- SwissProt: Q9NVM4 Human
- SwissProt: Q922X9 Mouse
- SwissProt: Q5U4E8 Rat
- Unigene: 640229 Human
see all -
別名
- [Myelin basic protein]-arginine N-methyltransferase PRMT7 antibody
- ANM7_HUMAN antibody
- FLJ10640 antibody
see all
画像
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Anti-PRMT7 antibody (ab126965) at 1/2000 dilution + PC3 whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 78 kDa
7.5% SDS PAGE -
ab126965, at 1/500 dilution, staining PRMT7 in Paraffin-embedded Rat RT2 xenograft tissue by Immunohistochemistry.
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ab126965, at 1/500 dilution, staining PRMT7 in Paraffin-embedded Human Hela xenograft tissue by Immunohistochemistry.
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ab126965, at 1/500 dilution, staining PRMT7 in Paraffin-embedded Mouse C2C12 xenograft tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab126965 は 1 報の論文で使用されています。
- Gurung B et al. Menin epigenetically represses Hedgehog signaling in MEN1 tumor syndrome. Cancer Res 73:2650-8 (2013). WB . PubMed: 23580576