Anti-PRMT1 抗体 (ab73246)
Key features and details
- Rabbit polyclonal to PRMT1
- Suitable for: ICC, IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PRMT1 antibody
PRMT1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PRMT1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC, IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Dog, Non human primates -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PRMT1.
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ポジティブ・コントロール
- ICC: HeLa cells. IP: HepG2 whole cell extract. IHC-P: Human hippocampus tissue. WB: Caco-2 and SW480 whole cell lysate. Mouse tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73246の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 1 - 5 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml.
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WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 42, 39 kDa (predicted molecular weight: 42 kDa).
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IP |
Use at an assay dependent concentration.
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特記事項 |
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ICC
Use a concentration of 1 - 5 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42, 39 kDa (predicted molecular weight: 42 kDa). |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the protein arginine N-methyltransferase family. -
細胞内局在
Nucleus. Cytoplasm > cytosol. - Information by UniProt
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参照データベース
- Entrez Gene: 520388 Cow
- Entrez Gene: 3276 Human
- Entrez Gene: 15469 Mouse
- Entrez Gene: 60421 Rat
- Omim: 602950 Human
- SwissProt: Q99873 Human
- SwissProt: Q9JIF0 Mouse
- SwissProt: Q63009 Rat
see all -
別名
- ANM 1 antibody
- ANM1 antibody
- ANM1_HUMAN antibody
see all
画像
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All lanes : Anti-PRMT1 antibody (ab73246) at 1 µg/ml
Lane 1 : Caco-2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab76828)
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab76999)
Lane 3 : Thymus (Mouse) Tissue Lysate (ab76823)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa (possible non-specific binding), 38 kDa (possible isoform), 90 kDa (possible non-specific binding)
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% milk before being incubated with ab73246 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
This antibody was raised against an immunogen that is predicted to recognize isoforms 1,2,3 and 4 of human PRMT1. The predicted molecular weights of isoforms 1,2,3 and 4 are 41kDa, 39kDa, 39kDa and 40kDa respectively.
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PRMT1 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to PRMT1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73246.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 42kDa: PRMT1; non specific - 30kDa: We are unsure as to the identity of this extra band. -
ab73246 staining PRMT1 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab73246 at 1 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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IHC image of PRMT1 staining in Human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73246, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab73246 は 17 報の論文で使用されています。
- Roth F et al. Assessment of PABPN1 nuclear inclusions on a large cohort of patients and in a human xenograft model of oculopharyngeal muscular dystrophy. Acta Neuropathol 144:1157-1170 (2022). PubMed: 36197469
- Musiani D et al. PRMT1 Is Recruited via DNA-PK to Chromatin Where It Sustains the Senescence-Associated Secretory Phenotype in Response to Cisplatin. Cell Rep 30:1208-1222.e9 (2020). PubMed: 31995759
- Spadotto V et al. PRMT1-mediated methylation of the microprocessor-associated proteins regulates microRNA biogenesis. Nucleic Acids Res 48:96-115 (2020). PubMed: 31777917
- Chen YY et al. Protein arginine methyltranferase-1 induces ER stress and epithelial-mesenchymal transition in renal tubular epithelial cells and contributes to diabetic nephropathy. Biochim Biophys Acta Mol Basis Dis 1865:2563-2575 (2019). PubMed: 31199999
- Chen C et al. PRMT1 potentiates chondrosarcoma development through activation of YAP activity. Mol Carcinog 58:2193-2206 (2019). PubMed: 31478263