Anti-Prickle 抗体 (ab15577)
Key features and details
- Rabbit polyclonal to Prickle
- Suitable for: IHC-P, ELISA
- Isotype: IgG
製品の概要
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製品名
Anti-Prickle antibody -
製品の詳細
Rabbit polyclonal to PrickleThis product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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由来種
Rabbit -
特異性
Immunogen sequence most homologous with Prickle 1, but is ~65% identical with Prickle 2 sequences, thus some reaction with Prickle 2 may be seen as well as with Prickle 1. -
種交差性
交差が予測される動物種: Mouse, Rat, Human, Xenopus laevis, Zebrafish
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免疫原
Synthetic peptide corresponding to Xenopus laevis Prickle aa 300-400 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab15578) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (2) |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ELISA |
Use at an assay dependent concentration. This antibody gave a positive result in ELISA against the immunizing peptide (ab15578).
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| 特記事項 |
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IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ELISA
Use at an assay dependent concentration. This antibody gave a positive result in ELISA against the immunizing peptide (ab15578). |
ターゲット情報
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関連性
Prickle is a LIM domain protein that appears to serve as a nuclear receptor for REST/NRSF, REST4, and possibly other transcription factors. The human protein is a 831 amino acid protein containing 3 N-terminal LIM domains and 3 C-terminal nuclear localization signals. It also contains 4 N-glycosylation sites, 2 PKA phosphorylation sites, and a C-terminal CIIS prenylation motif. -
細胞内局在
Nuclear Membrane -
参照データベース
- Entrez Gene: 144165 Human
- Entrez Gene: 106042 Mouse
- Entrez Gene: 315259 Rat
- Entrez Gene: 368249 Zebrafish
- Omim: 608500 Human
- SwissProt: Q14C83 Human
- SwissProt: Q96MT3 Human
- SwissProt: Q3U5C7 Mouse
see all -
別名
- FLJ31627 antibody
- FLJ31937 antibody
- MGC138902 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prickle antibody (ab15577)Image courtesy of an Abreview submitted by Antibody Solutionsab15577 (1/50) staining Prickle in paraffin-embbeded human uterus (smooth muscle). Tissues underwent formaldehyde fixation, heat mediated antigen retrieval and peroxidase blocking prior to staining of the sections. Please refer to Abreview for further experimental details.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab15577 は 4 報の論文で使用されています。
- Kotrbová A et al. WNT signaling inducing activity in ascites predicts poor outcome in ovarian cancer. Theranostics 10:537-552 (2020). PubMed: 31903136
- Gignac SJ et al. Robinow syndrome skeletal phenotypes caused by the WNT5AC83S variant are due to dominant interference with chondrogenesis. Hum Mol Genet 28:2395-2414 (2019). PubMed: 31032853
- Wan Y et al. Prickle1 regulates differentiation of frontal bone osteoblasts. Sci Rep 8:18021 (2018). PubMed: 30575813
- Song H et al. Androgen receptor drives hepatocellular carcinogenesis by activating enhancer of zeste homolog 2-mediated Wnt/ß-catenin signaling. EBioMedicine 35:155-166 (2018). PubMed: 30150059
