Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (ab234592)
製品の概要
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製品名
Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)
Prestained Protein Ladder ihc 試薬 製品一覧 -
アプリケーション
適用あり: WB, SDS-PAGEmore details -
特記事項
Prestained Protein Ladder ab234592 is a three-color protein standard with 10 pre-stained proteins covering a range of MW from 6.5-270 kDa. This prestained protein ladder allows you to monitor protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key product features:
- Broad range: 6.5-270 kDa
- Ready to use: The ladder is supplied in the gel loading buffer and is ready to use.
- Easily identified: Proteins are covalently coupled with a blue chromophore except three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa).
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Review other protein ladders in the unstained and prestained protein ladder guide.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
pH: 7.50
Preservative: 0.01% Sodium azide
Constituents: 1.37% Tris phosphate, 0.03% EDTA, 2% Sodium lauryl sulfate, 0.15% DTT, 33% Glycerol -
Concentration information loading...
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研究分野
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別名
- Molecular weight marker
- Prism protein ladder
- SDS PAGE protein ladder prestained
see all
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab234592の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 2.5 μl. |
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SDS-PAGE |
Use at an assay dependent concentration.
For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 µl per well for 15-well or 10-well mini-gel, respectively. |
特記事項 |
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WB
Use at an assay dependent concentration. For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 2.5 μl. |
SDS-PAGE
Use at an assay dependent concentration. For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 µl per well for 15-well or 10-well mini-gel, respectively. |
画像
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SDS-PAGE analysis with ab234592.
Gel 1: Tris-Glycine 4-20%
Gel 2: Bis-Tris 4-12% MES buffer.
The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab234592 は 1 報の論文で使用されています。
- Bhattacharya A et al. Oncogenes overexpressed in metastatic oral cancers from patients with pain: potential pain mediators released in exosomes. Sci Rep 10:14724 (2020). PubMed: 32895418