製品の概要

  • 製品名

    Prestained Protein Ladder – Broad molecular weight (10-245 kDa)
    Prestained Protein Ladder ihc 試薬 製品一覧
  • 製品の詳細

    Prestained Protein Ladder - Broad molecular weight (10-245 kDa)
  • アプリケーション

    適用あり: SDS-PAGE, WBmore details
  • 特記事項

    Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).

    This prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The protein ladder is supplied in gel loading buffer and is ready to use.

    Key product features:

    • Broad range: 10-245 kDa
    • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
    • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
    • Sharp bands
    • Recommended loading: ~1.5 - 2.5µl

     

    Review other protein ladders in the unstained and prestained protein ladder guide.

    This product was previously called Prism Ultra Protein Ladder (10-245 kDa).

    Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Please see notes section.
  • バッファー

    Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% SDS, 0.02% DTT, 15% Glycerol
  • Concentration information loading...
  • 研究分野

  • 別名

    • Molecular weight marker
    • Prism protein ladder
    • SDS PAGE protein ladder prestained
    • WB prestained protein ladder
    • Western blot prestained protein ladder
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab116028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
SDS-PAGE Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
WB Use at an assay dependent concentration. ~1.5-2.5 µl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.

画像

  • Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (10%) MOPS buffer, Gel 3: Bis-Tris (10%) MES buffer. Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

参考文献

This product has been referenced in:

  • Martinotti S  et al. Honey-Mediated Wound Healing: H2O2 Entry through AQP3 Determines Extracellular Ca2+ Influx. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30754672) »
  • Singh AK  et al. Classification of Clinical Isolates of Klebsiella pneumoniae Based on Their in vitro Biofilm Forming Capabilities and Elucidation of the Biofilm Matrix Chemistry With Special Reference to the Protein Content. Front Microbiol 10:669 (2019). Read more (PubMed: 31019496) »
See all 7 Publications for this product

レビューと Q&A

1-4 of 4 Abreviews or Q&A

Prestained ladder works at LICOR

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The ladder works perfectly at 2.5 ul (down to 1 ul) and also shows nice crisps bands not only on the nitrocellulose membrane but also when analysed at the LICOR. Same if not even a touch better results then comparable ladders from more expensive competitors.

Sebastian Bohm

Verified customer

投稿 Jun 22 2018

One of the best Pre stained protein ladder

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I have used 1.8 ul per well and get a clear band on transfer to membrane.

Vicky

Verified customer

投稿 Oct 10 2016

Answer

The apparent sizes will be the same as the ones in the second gel on the online datasheet, which is a 10% Bis-Tris gel, MOPS running buffer. The distribution will be different, with a little more separation of the higher molecular weight proteins at the 4% end of your gel compared to the 10% gel on the datasheet.

Read More

Question
Answer

I can confirm that ab116027, ab116028, and ab116029 do not contain histone tagged proteins.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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