Anti-PRDM1/Blimp1 抗体 (ab106766)
Key features and details
- Goat polyclonal to PRDM1/Blimp1
- Suitable for: WB, Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PRDM1/Blimp1 antibody
PRDM1/Blimp1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PRDM1/Blimp1 -
由来種
Goat -
アプリケーション
適用あり: WB, Flow Cyt (Intra), ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig
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免疫原
Synthetic peptide:
C-DISDNADRLEDVED
, corresponding to internal sequence amino acids 734-747 of Human PRDM1/Blimp1 (NP_001189.2) -
ポジティブ･コントロール
- Flow Cyt (Intra): A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106766の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).
1 hour primary incubation is recommended for this product. |
|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
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| ICC/IF |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa). 1 hour primary incubation is recommended for this product. |
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). -
配列類似性
Belongs to the class V-like SAM-binding methyltransferase superfamily.
Contains 4 C2H2-type zinc fingers.
Contains 1 SET domain. -
翻訳後修飾
Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 538384 Cow
- Entrez Gene: 481947 Dog
- Entrez Gene: 639 Human
- Entrez Gene: 12142 Mouse
- Entrez Gene: 100154284 Pig
- Entrez Gene: 309871 Rat
- Omim: 603423 Human
- SwissProt: O75626 Human
see all -
別名
- B Lymphocyte Induced Maturation Protein 1 antibody
- Beta interferon gene positive regulatory domain I binding factor antibody
- Beta-interferon gene positive regulatory domain I-binding factor antibody
see all
画像
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Western blot - Anti-PRDM1/Blimp1 antibody (ab106766)All lanes : Anti-PRDM1/Blimp1 antibody (ab106766) at 0.1 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : K562 nuclear lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 92 kDa -
Flow Cytometry (Intracellular) - Anti-PRDM1/Blimp1 antibody (ab106766)Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab106766 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-PRDM1/Blimp1 antibody (ab106766)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab106766 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Western blot - Anti-PRDM1/Blimp1 antibody (ab106766)Anti-PRDM1/Blimp1 antibody (ab106766) at 0.3 µg/ml + A431 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 92 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab106766 は 1 報の論文で使用されています。
- Kim SJ et al. Regulation of dendritic cell activation by microRNA let-7c and BLIMP1. J Clin Invest N/A:N/A (2013). ChIP ; Mouse . PubMed: 23298838
