Anti-PRDM16 抗体 (ab106410)
Key features and details
- Rabbit polyclonal to PRDM16
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PRDM16 antibody
PRDM16 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PRDM16 -
由来種
Rabbit -
特異性
At least four isoforms of PRDM16 are known to exist; ab106410 will recognize all four. -
アプリケーション
適用あり: ICC, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human PRDM16 (C terminal). The immunogen is located within amino acids 1120 - 1170 of PRDM16.
Database link: Q9HAZ2 -
ポジティブ･コントロール
- WB: K562 cell lysate IHC-P: Human brain tissue ICC: Human brain cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106410の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use a concentration of 20 µg/ml.
|
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| IHC-P | (3) |
Use a concentration of 2 µg/ml.
|
| WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 140 kDa.
|
| 特記事項 |
|---|
|
ICC
Use a concentration of 20 µg/ml. |
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IHC-P
Use a concentration of 2 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 140 kDa. |
ターゲット情報
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機能
Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation. -
組織特異性
Expressed in uterus and kidney. -
関連疾患
Note=A chromosomal aberration involving PRDM16 is found in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Reciprocal translocation t(1;3)(p36;q21). Isoform 4 is specifically expressed in adult T-cell leukemia. -
配列類似性
Contains 10 C2H2-type zinc fingers.
Contains 1 SET domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 63976 Human
- Omim: 605557 Human
- SwissProt: Q9HAZ2 Human
- Unigene: 99500 Human
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別名
- CMD1LL antibody
- KIAA1675 antibody
- LVNC8 antibody
see all
画像
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Western blot - Anti-PRDM16 antibody (ab106410)Anti-PRDM16 antibody (ab106410) at 1 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 140 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM16 antibody (ab106410)Paraffin-embedded human brain tissue stained for PRDM16 using ab106410 at 2 µg/ml in immunohistochemical analysis.
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Immunocytochemistry - Anti-PRDM16 antibody (ab106410)Image is courtesy of an Abreview submitted by Lena Appelsvedab106410 staining RAP1 in Human Brown adipocytes from supraclavicular fat tissue by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum, 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (2 ug/ml in 10% serum, 1% BSA, 0.3% Triton X100, PBS) for 16 hours at 4°C. A Cy3®-conjugated Donkey F(ab')2 anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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Immunocytochemistry - Anti-PRDM16 antibody (ab106410)Immunocytochemical analysis of PRDM16 in Human Brain cells using ab106410 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (56)
ab106410 は 56 報の論文で使用されています。
- Peng WQ et al. l-Theanine Activates the Browning of White Adipose Tissue Through the AMPK/a-Ketoglutarate/Prdm16 Axis and Ameliorates Diet-Induced Obesity in Mice. Diabetes 70:1458-1472 (2021). PubMed: 33863801
- Zhao D et al. Curcumin improves adipocytes browning and mitochondrial function in 3T3-L1 cells and obese rodent model. R Soc Open Sci 8:200974 (2021). PubMed: 33959308
- Biferali B et al. Prdm16-mediated H3K9 methylation controls fibro-adipogenic progenitors identity during skeletal muscle repair. Sci Adv 7:N/A (2021). PubMed: 34078594
- Maharjan BR et al. Exercise induces favorable metabolic changes in white adipose tissue preventing high-fat diet obesity. Physiol Rep 9:e14929 (2021). PubMed: 34405572
- Liang X et al. Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis. Protein Cell N/A:N/A (2020). PubMed: 32797353