リコンビナントRabMAb

Anti-PR3 抗体 [EPR23253-35] - BSA and Azide free (ab270610)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
  • Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
  • Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23253-35] to PR3 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Human

製品の概要

  • 製品名

    Anti-PR3 antibody [EPR23253-35] - BSA and Azide free
    PR3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23253-35] to PR3 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human bone marrow, lung, spleen and placenta tissue; Mouse lung and spleen tissue. ICC/IF: Mouse bone marrow cells.

  • 特記事項

    ab270610 is the carrier-free version of ab270441. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC/IF
Mouse
IHC-P
Human
アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for Flow Cyt,IP or WB.

    • ターゲット情報

    • 機能

      Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.

    • 配列類似性

      Belongs to the peptidase S1 family. Elastase subfamily.
      Contains 1 peptidase S1 domain.
    • Information by UniProt

    • 参照データベース

      • 別名

        • ACPA antibody
        • AGP 7 antibody
        • AGP7 antibody
        • AGP7 serine proteinase antibody
        • Azurophil Granule Protein 7 antibody
        • C ANCA antibody
        • C ANCA antigen antibody
        • C-ANCA antigen antibody
        • CANCA antibody
        • EC 3.4.21.76 antibody
        • Leukocyte proteinase 3 antibody
        • MBN antibody
        • MBT antibody
        • MBT WEGENER AUTOANTIGEN antibody
        • Myeloblastin antibody
        • Neutrophil proteinase 4 antibody
        • NP 4 antibody
        • NP-4 antibody
        • NP4 antibody
        • P29 antibody
        • PR 3 antibody
        • PR-3 antibody
        • PR3 antibody
        • Proteinase 3 antibody
        • Proteinase3 antibody
        • PRTN 3 antibody
        • Prtn3 antibody
        • PRTN3_HUMAN antibody
        • Serine proteinase neutrophil Wegener granulomatosis autoantigen antibody
        • Serine proteinase, neutrophil antibody
        • Wegener autoantigen antibody
        • Wegener granulomatosis autoantigen antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)

        Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on human bone marrow (PMID: 22247758).The section was incubated with ab270441 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)

        Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PR3 with ab270441 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in mouse spleen. The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

      • Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
        Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)

        Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse bone marrow or EL4 cells labelling PR3 with ab270441 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in a subset of mouse bone marrow cells. Negative control: EL4 (PMID: 9211743). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

        Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)

        Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PR3 with ab270441 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in mouse lung (PMID: 22247758).The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)

        Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human placenta.The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)

        Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human spleen.The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)

        Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human lung (PMID: 22247758).The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

      • Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)
        Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (ab270610)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab270610 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab270610 は論文での使用が確認できていません。

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