Anti-PPP2R5D 抗体 [EPR15617-50] (ab188325)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15617-50] to PPP2R5D
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PPP2R5D antibody [EPR15617-50]
PPP2R5D 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15617-50] to PPP2R5D -
由来種
Rabbit -
特異性
Based on sequence analysis, ab188325 recognizes 3 isoforms with the predicted MWs of 58kDa, 66kDa and 70kDa, respectively -
アプリケーション
適用あり: WB, IHC-P, IPmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: A431, MCF7, C6, HepG2, HeLa, Jurkat and 293T cell lysates. IHC-P: Human brain and astrocytoma tissues. IP: 293T cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15617-50 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab188325の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 70 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/40 - 1/60.
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特記事項 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 70 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/40 - 1/60. |
ターゲット情報
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機能
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. -
組織特異性
Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain. -
配列類似性
Belongs to the phosphatase 2A regulatory subunit B56 family. -
細胞内局在
Cytoplasm. Nucleus. Nuclear in interphase, nuclear during mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 5528 Human
- Entrez Gene: 21770 Mouse
- Entrez Gene: 363193 Rat
- Omim: 601646 Human
- SwissProt: Q14738 Human
- Unigene: 533308 Human
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別名
- 2A5D_HUMAN antibody
- B'delta antibody
- B56D antibody
see all
画像
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All lanes : Anti-PPP2R5D antibody [EPR15617-50] (ab188325) at 1/10000 dilution
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : PPP2R5D knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 60-65 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab188325 observed at 60-65 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab188325 was shown to react with PPP2R5D in wild-type A431 cells in western blot with loss of signal observed in PPP2R5D knockout sample. Wild-type and PPP2R5D knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab188325 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PPP2R5D with ab188325 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-PPP2R5D antibody [EPR15617-50] (ab188325) at 1/50000 dilution
Lane 1 : C6 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 70 kDa -
Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling PPP2R5D with ab188325 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Western blot analysis of PPP2R5D in 293T cell lysate immunoprecipitated with ab188325 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1500 was used as secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab188325 は論文での使用が確認できていません。