Anti-PPP2R5B 抗体 (ab1366)
Key features and details
- Goat polyclonal to PPP2R5B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PPP2R5B antibody
PPP2R5B 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PPP2R5B -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog
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免疫原
Synthetic peptide: QRLTPQVAASGGQS, corresponding to C terminal amino acids 484-497 of Human PPP2R5B.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab1366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
WB: Use at a concentration of 2 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 on the 'Thr-308' and 'Ser-373' residues. -
組織特異性
Highest expression in brain. -
配列類似性
Belongs to the phosphatase 2A regulatory subunit B56 family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5526 Human
- Entrez Gene: 225849 Mouse
- Entrez Gene: 309179 Rat
- NCBI: 5453952 Human
- Omim: 601644 Human
- SwissProt: Q15173 Human
- Unigene: 75199 Human
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別名
- 2A5B_HUMAN antibody
- B56B antibody
- Beta isoform of regulatory subunit B56 protein phosphatase 2A antibody
see all
画像
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Western blot - Anti-PPP2R5B antibody (ab1366)Anti-PPP2R5B antibody (ab1366) at 2 µg/ml + Human cerebellum lysate at 35 µg
Predicted band size: 55 kDa
Observed band size: 55 kDa
データシートおよび資料
参考文献 (1)
ab1366 は 1 報の論文で使用されています。
- DeGrande ST et al. Molecular mechanisms underlying cardiac protein phosphatase 2A regulation in heart. J Biol Chem 288:1032-46 (2013). WB ; Human, Mouse, Rat, Dog . PubMed: 23204520
