Anti-PPP2R5B 抗体 (ab1366)

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Western blot - Anti-PPP2R5B antibody (ab1366)

    Key features and details

    • Goat polyclonal to PPP2R5B
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-PPP2R5B antibody
      PPP2R5B 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to PPP2R5B

    • 由来種

      Goat

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat, Dog

    • 免疫原

      Synthetic peptide: QRLTPQVAASGGQS, corresponding to C terminal amino acids 484-497 of Human PPP2R5B.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab1366の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    特記事項
  • 追加情報
    ELISA: Detection limit dilution 1/2000.
    WB: Use at a concentration of 2 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能

      The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 on the 'Thr-308' and 'Ser-373' residues.

    • 組織特異性

      Highest expression in brain.

    • 配列類似性

      Belongs to the phosphatase 2A regulatory subunit B56 family.

    • 細胞内局在

      Cytoplasm.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 2A5B_HUMAN antibody
        • B56B antibody
        • Beta isoform of regulatory subunit B56 protein phosphatase 2A antibody
        • FLJ35411 antibody
        • PP2A B subunit B' beta isoform antibody
        • PP2A B subunit B56 beta isoform antibody
        • PP2A B subunit isoform B' beta antibody
        • PP2A B subunit isoform B''-beta antibody
        • PP2A B subunit isoform B56-beta antibody
        • PP2A B subunit isoform PR61-beta antibody
        • PP2A B subunit isoform R5-beta antibody
        • PP2A B subunit PR61 beta isoform antibody
        • PP2A B subunit R5 beta isoform antibody
        • PPP2R5B antibody
        • PR 61B antibody
        • PR61B antibody
        • Protein phosphatase 2 regulatory subunit B (B56) beta antibody
        • Protein phosphatase 2 regulatory subunit B (B56) beta isoform antibody
        • Protein phosphatase 2 regulatory subunit B' beta antibody
        • Protein phosphatase 2 regulatory subunit B' beta isoform antibody
        • Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit beta isoform antibody
        • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform antibody
        see all

      画像

      • Western blot - Anti-PPP2R5B antibody (ab1366)
        Western blot - Anti-PPP2R5B antibody (ab1366)
        Anti-PPP2R5B antibody (ab1366) at 2 µg/ml + Human cerebellum lysate at 35 µg

        Predicted band size: 55 kDa
        Observed band size: 55 kDa

      参考文献 (1)

      ab1366 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab1366 は 1 報の論文で使用されています。

      • DeGrande ST  et al. Molecular mechanisms underlying cardiac protein phosphatase 2A regulation in heart. J Biol Chem 288:1032-46 (2013). WB ; Human, Mouse, Rat, Dog . PubMed: 23204520

      レビューと Q&A

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      Immunocytochemistry/ Immunofluorescence abreview for Anti-PPP2R5B antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HEK293T)
      Permeabilization
      Yes - 0.1% Triton X-100
      Specification
      HEK293T
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

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      投稿 Mar 31 2017

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