Anti-PPP2R5B 抗体 (ab1366)
製品の概要
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製品名
Anti-PPP2R5B antibody
PPP2R5B 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PPP2R5B -
由来種
Goat -
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog
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免疫原
Synthetic peptide: QRLTPQVAASGGQS, corresponding to C terminal amino acids 484-497 of Human PPP2R5B.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab1366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | ||
| WB |
追加情報
ELISA: Detection limit dilution 1/2000.
WB: Use at a concentration of 2 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 2 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 on the 'Thr-308' and 'Ser-373' residues. -
組織特異性
Highest expression in brain. -
配列類似性
Belongs to the phosphatase 2A regulatory subunit B56 family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5526 Human
- Entrez Gene: 225849 Mouse
- Entrez Gene: 309179 Rat
- NCBI: 5453952 Human
- Omim: 601644 Human
- SwissProt: Q15173 Human
- Unigene: 75199 Human
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別名
- 2A5B_HUMAN antibody
- B56B antibody
- Beta isoform of regulatory subunit B56 protein phosphatase 2A antibody
see all
画像
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Western blot - Anti-PPP2R5B antibody (ab1366)Anti-PPP2R5B antibody (ab1366) at 2 µg/ml + Human cerebellum lysate at 35 µg
Predicted band size: 55 kDa
Observed band size: 55 kDa
プロトコール
データシートおよび資料
参考文献
This product has been referenced in:
- DeGrande ST et al. Molecular mechanisms underlying cardiac protein phosphatase 2A regulation in heart. J Biol Chem 288:1032-46 (2013). WB ; Human, Mouse, Rat, Dog . Read more (PubMed: 23204520) »
