リコンビナントRabMAb

Anti-PPM1D/WIP1 抗体 [EPR22960-39] - BSA and Azide free (ab269864)

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Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free (ab269864)
  • Flow Cytometry - Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free (ab269864)
  • Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free (ab269864)
  • Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free (ab269864)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22960-39] to PPM1D/WIP1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free
    PPM1D/WIP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22960-39] to PPM1D/WIP1 - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    The antibody detects specifically PPM1D430.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: MCF7, Jurkat, A549, F9, HCT 116, RAW 264.7, PC-12 and NIH/3T3 whole cell lysate, Human testis and lung cancer tissue lysates, Mouse and Rat spleen tissue lysates. ICC/IF: MCF7, HCT-116 cells. Flow Cyt: MCF7 cell.

  • 特記事項

    ab269864 is the carrier-free version of ab234439. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC/IF
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 67 kDa.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 67 kDa.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins.

  • 配列類似性

    Belongs to the PP2C family.
    Contains 1 PP2C-like domain.
  • Information by UniProt

  • 参照データベース

    • 別名

      • EC 3.1.3.16 antibody
      • p53 induced protein phosphatase 1 antibody
      • p53-induced protein phosphatase 1 antibody
      • PP2C delta antibody
      • PP2C-delta antibody
      • PP2CD antibody
      • Ppm1d antibody
      • PPM1D_HUMAN antibody
      • Protein phosphatase 1D antibody
      • protein phosphatase 1D magnesium-dependent delta isoform antibody
      • Protein phosphatase 2C delta isoform antibody
      • Protein phosphatase 2C isoform delta antibody
      • Protein phosphatase magnesium dependent 1 delta antibody
      • Protein phosphatase magnesium-dependent 1 delta antibody
      • Protein phosphatase Mg2+/Mn2+ dependent 1D antibody
      • Protein phosphatase Wip1 antibody
      • Wild type p53 induced phosphatase 1 antibody
      • WIP 1 antibody
      • WIP1 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free (ab269864)
      Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free (ab269864)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in MCF7 cells. Tubulin is detected with Anti-alpha Tubulin antibody (Alexa Fluor® 594) ab195889 at 1/200 dilution (Red). Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234439).

    • Flow Cytometry - Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free (ab269864)
      Flow Cytometry - Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free (ab269864)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234439).

    • Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free (ab269864)
      Immunocytochemistry/ Immunofluorescence - Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free (ab269864)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HCT-116 (Human colorectal carcinoma cell line) cells labelling PPM1D430/WIP1s with ab234439 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HCT-116 cells. Tubulin is detected with Anti-alpha Tubulin antibody (Alexa Fluor® 594) ab195889 at 1/200 dilution (Red). Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234439).

    • Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free (ab269864)
      Anti-PPM1D/WIP1 antibody [EPR22960-39] - BSA and Azide free (ab269864)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab269864 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab269864 は論文での使用が確認できていません。

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