PPAR delta Transcription Factor Assay Kit (ab133106)
製品の概要
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製品名PPAR delta Transcription Factor Assay Kit
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検出方法Colorimetric
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サンプルの種類Adherent cells, Suspension cells, Nuclear Extracts
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アッセイタイプSemi-quantitative
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種交差性交差種: Mouse, Human
交差が予測される動物種: Mammals
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製品の概要
Abcam's PPAR delta Transcription Factor Assay Kit (ab133106) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.
A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR delta is detected by addition of specific primary antibody directed against PPAR delta. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133106 detects human PPAR delta.
This assay does not cross-react with PPAR gamma 1 and PPAR gamma 2 or PPAR alpha.
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特記事項
Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.
PPAR delta is the least understood; however, identification of a high-affinity PPAR delta synthetic ligand suggests a potential role for this receptor in lipid metabolism and obesity. PPAR delta is ubiquitously expressed, but it is particularly abundant in tissues such as liver, intestine, kidney, abdominal adipose, and skeletal muscle.
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試験プラットフォームMicroplate reader
製品の特性
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保存方法Please refer to protocols.
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内容 96 tests 96-Well Plate Cover 1 unit Polysorbate 20 1 vial Transcription Factor Antibody Binding Buffer (10X) 1 x 3ml Transcription Factor Binding Assay Buffer (4X) 1 x 3ml Transcription Factor Developing Solution 1 x 12ml Transcription Factor Goat Anti-Rabbit HRP Conjugate 1 x 100µl Transcription Factor PPAR 96-Well Strip Plate 1 unit Transcription Factor PPAR Competitor dsDNA 1 vial Transcription Factor PPARδ Positive Control 1 vial Transcription Factor PPARδ Primary Antibody 1 vial Transcription Factor Reagent A 1 x 120µl Transcription Factor Stop Solution 1 x 12ml Wash Buffer Concentrate (400X) 1 x 5ml -
研究分野
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機能Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
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組織特異性Ubiquitous with maximal levels in placenta and skeletal muscle.
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配列類似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
細胞内局在Nucleus.
- Information by UniProt
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別名
- FAAR
- MGC3931
- NR1C2
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参照データベース
- Entrez Gene: 5467 Human
- Entrez Gene: 19015 Mouse
- Omim: 600409 Human
- SwissProt: Q03181 Human
- SwissProt: P35396 Mouse
- Unigene: 696032 Human
- Unigene: 328914 Mouse
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sELISA pair antibody
画像
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Functional Studies - PPAR delta Transcription Factor Assay Kit (ab133106)Panel A: Increasing amounts of positive control (total lysate) are assayed for PPAR delta DNA-binding activity using ab133106.
Panel B: PPAR delta DNA-binding assays are performed in the presence of competitive dsDNA. The decrease in signal caused by addition of competitive dsDNA confirms the assay specificity.
プロトコール
参考文献
This product has been referenced in:
- Sharif O et al. The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia. PLoS Pathog 10:e1004167 (2014). Functional Studies ; Mouse . Read more (PubMed: 24945405) »
