Anti-PPAR alpha (phospho S12) 抗体 (ab3484)
Key features and details
- Rabbit polyclonal to PPAR alpha (phospho S12)
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PPAR alpha (phospho S12) antibody
PPAR alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PPAR alpha (phospho S12) -
由来種
Rabbit -
特異性
The antibody is expected to bind both phospho and non phospho forms. -
アプリケーション
適用あり: ICC/IF, WB, Flow Cytmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Guinea pig, Dog -
免疫原
Synthetic peptide corresponding to Mouse PPAR alpha aa 8-19.
Sequence: ICPLSpPLEADDL
(Peptide available asab4998) -
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | Use at an assay dependent concentration. | |
WB | 1/100 - 1/1000. Predicted molecular weight: 52 kDa. | |
Flow Cyt | Use 3-5µg for 106 cells. |
ターゲット情報
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機能
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. -
組織特異性
Skeletal muscle, liver, heart and kidney. -
配列類似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 403654 Dog
- Entrez Gene: 100135604 Guinea pig
- Entrez Gene: 5465 Human
- Entrez Gene: 19013 Mouse
- Omim: 170998 Human
- SwissProt: Q95N78 Dog
- SwissProt: O35507 Guinea pig
- SwissProt: Q07869 Human
see all -
別名
- hPPAR antibody
- MGC2237 antibody
- MGC2452 antibody
see all
画像
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All lanes : Anti-PPAR alpha (phospho S12) antibody (ab3484) at 1/200 dilution
Lane 1 : C2C12 cell lysate
Lane 2 : NIH-3T3 cell lysate
Lane 3 : 3T3-L1 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa -
Immunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-PPAR alpha pSer12 polyclonal antibody (ab3484) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of 3T3-L1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-PPAR alpha pSer12 polyclonal antibody (ab3484) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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All lanes : Anti-PPAR alpha (phospho S12) antibody (ab3484) at 1/1000 dilution
Lane 1 : U-87 MG with Skimmed milk
Lane 2 : MCF7 with Skimmed milk
Lane 3 : MDA-MB-231 with Skimmed milk
Lane 4 : C2C12 with Skimmed milk
Lane 5 : Hep G2 with Skimmed milk
Lane 6 : NIH/3T3 with Skimmed milk
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat anti-rabbit IgG (H+L) at 1/2500 dilution
Predicted band size: 52 kDa -
Immunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-PPAR alpha pSer12 polyclonal antibody (ab3484) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-PPAR alpha (phospho S12) antibody (ab3484)This image is courtesy of an anonymous Abreviewab3484 staining PPAR alpha (phospho S12) in Mouse neuronal cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 18 hours at 4°C. A Cy2®-conjugated Donkey anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
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ab3484 staining PPAR alpha (phospho S12) in MCF7 cells by Flow Cytometry. The sample was incubated with the primary antibody (3-5 ug/million cells in 2.5% BSA) for 2 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit was used as the secondary antibody (1/400). Red histogram represents ab3484, pink histogram represents isotype control, purple histogram represents unstained control and green histogram represents no primary antibody control.
プロトコール
データシートおよび資料
参考文献 (15)
ab3484 は 15 報の論文で使用されています。
- Wang H et al. Phytanic acid activates PPARa to promote beige adipogenic differentiation of preadipocytes. J Nutr Biochem 67:201-211 (2019). PubMed: 30951974
- Sun YL et al. Mechanism of pomegranate ellagic polyphenols reducing insulin resistance on gestational diabetes mellitus rats. Am J Transl Res 11:5487-5500 (2019). PubMed: 31632524
- Barjaktarovic Z et al. Hyperacetylation of Cardiac Mitochondrial Proteins Is Associated with Metabolic Impairment and Sirtuin Downregulation after Chronic Total Body Irradiation of ApoE -/- Mice. Int J Mol Sci 20:N/A (2019). PubMed: 31652604
- Xiao Y et al. Neurotensin contributes to pediatric intestinal failure-associated liver disease via regulating intestinal bile acids uptake. EBioMedicine 35:133-141 (2018). PubMed: 30104181
- Yang BY et al. Different physiological roles of insulin receptors in mediating nutrient metabolism in zebrafish. Am J Physiol Endocrinol Metab 315:E38-E51 (2018). PubMed: 29351486