Anti-PPAR alpha 抗体 (ab61182)
Key features and details
- Rabbit polyclonal to PPAR alpha
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PPAR alpha antibody
PPAR alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PPAR alpha -
由来種
Rabbit -
特異性
ab61182 detects PPAR alpha protein, (SwissProt Q07869). -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic non-phosphopeptide derived from human PPAR alpha around the phosphorylation site of serine 21 (L-E-SP-P-L).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab61182の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
1/100 - 1/500.
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WB |
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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特記事項 |
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ICC/IF
1/100 - 1/500. |
WB
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
ターゲット情報
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機能
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. -
組織特異性
Skeletal muscle, liver, heart and kidney. -
配列類似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5465 Human
- Entrez Gene: 19013 Mouse
- Omim: 170998 Human
- SwissProt: Q07869 Human
- SwissProt: P23204 Mouse
- Unigene: 103110 Human
- Unigene: 710044 Human
- Unigene: 212789 Mouse
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別名
- hPPAR antibody
- MGC2237 antibody
- MGC2452 antibody
see all
画像
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ab61182 at 1/100 - 1/500 dilution staining PPAR alpha in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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All lanes : Anti-PPAR alpha antibody (ab61182) at 1/500 dilution
Lane 1 : NIH 3T3 cell extracts
Lane 2 : NIH 3T3 cell extracts with the immunising peptide
Predicted band size: 52 kDa
Observed band size: 52 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab61182 は 6 報の論文で使用されています。
- Chen HX et al. Garcinia Biflavonoid 1 Improves Lipid Metabolism in HepG2 Cells via Regulating PPARα. Molecules 27:N/A (2022). PubMed: 35335339
- Mannino F et al. Atherosclerosis Plaque Reduction by Lycopene Is Mediated by Increased Energy Expenditure through AMPK and PPARα in ApoE KO Mice Fed with a High Fat Diet. Biomolecules 12:N/A (2022). PubMed: 35883529
- Wang X et al. Therapeutic Effects of Retinoic Acid in Lipopolysaccharide-Induced Cardiac Dysfunction: Network Pharmacology and Experimental Validation. J Inflamm Res 15:4963-4979 (2022). PubMed: 36105385
- Zhang B et al. Atorvastatin promotes AMPK signaling to protect against high fat diet-induced non-alcoholic fatty liver in golden hamsters. Exp Ther Med 19:2133-2142 (2020). PubMed: 32104276
- Li H et al. Gypenosides regulate farnesoid X receptor-mediated bile acid and lipid metabolism in a mouse model of non-alcoholic steatohepatitis. Nutr Metab (Lond) 17:34 (2020). PubMed: 32377219
- Azar S et al. Reversal of diet-induced hepatic steatosis by peripheral CB1 receptor blockade in mice is p53/miRNA-22/SIRT1/PPARa dependent. Mol Metab 42:101087 (2020). PubMed: 32987186