Anti-PP2A alpha + beta 抗体 (ab1309)

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製品の概要

  • 製品名

    Anti-PP2A alpha + beta antibody
    PP2A alpha + beta 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to PP2A alpha + beta

  • 由来種

    Goat

  • 特異性

    This antibody will recognise both alpha and beta isoforms, which are almost identical.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Dog, Pig

  • 免疫原

    Synthetic peptide corresponding to Human PP2A alpha + beta aa 297-309 (C terminal).
    Sequence:

    C-EPHVTRRTPDYFL


    (Peptide available as ab22802)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: Recombinant Human PP2A-alpha protein (ab114424) can be used as a positive control; Mouse/Rat/Pig brain whole cell lysate in RIPA buffer.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.03 - 3 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).Can be blocked with Human PP2A alpha + beta peptide (ab22802).

ターゲット情報

  • 機能

    PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.

  • 配列類似性

    Belongs to the PPP phosphatase family. PP-1 subfamily.

  • 翻訳後修飾

    Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
    Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.

  • 細胞内局在

    Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • PP2A A antibody
    • PP2A alpha antibody
    • PP2A B antibody
    • PP2A beta antibody
    • PP2A-alpha antibody
    • PP2A-beta antibody
    • PP2AA_HUMAN antibody
    • PP2Aalpha antibody
    • PP2AB_HUMAN antibody
    • PP2Abeta antibody
    • PP2Ac antibody
    • PP2CA antibody
    • PP2Calpha antibody
    • PP2CB antibody
    • PPP2CA antibody
    • PPP2CB antibody
    • Protein phosphatase 2 catalytic subunit alpha isoform antibody
    • Protein phosphatase 2 catalytic subunit beta isoform antibody
    • Protein phosphatase type 2A catalytic subunit antibody
    • Replication protein C antibody
    • RP C antibody
    • RP-C antibody
    • RPC antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
    • Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody
    • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
    see all

画像

  • Western blot - Anti-PP2A alpha + beta antibody (ab1309)
    Western blot - Anti-PP2A alpha + beta antibody (ab1309)
    All lanes : Anti-PP2A alpha + beta antibody (ab1309) at 0.03 µg/ml

    Lane 1 : Mouse brain whole cell lysate in RIPA buffer
    Lane 2 : Rat brain whole cell lysate in RIPA buffer
    Lane 3 : Pig brain whole cell lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Primary incubation was 1 hour.

  • Western blot - Anti-PP2A alpha + beta antibody (ab1309)
    Western blot - Anti-PP2A alpha + beta antibody (ab1309)
    Anti-PP2A alpha + beta antibody (ab1309) at 0.05 µg/ml + Human Cerebellum lysates at 35 µg

    Developed using the ECL technique.

    Predicted band size: 36 kDa



    Primary incubated for 1 hour.

プロトコール

参考文献

ab1309 has not yet been referenced specifically in any publications.

ab1309 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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Question

We have a customer who has purchased this antibody. She is studying rat liver and skeletal muscle via WEstern blot. These samples should be postiive for PPP2CA. She loaded 50 ugs of these extracts on her gel and used ponceau S to verify transfer. After a 1:500 dilution and incubation for 2 hours, she washed with TBST and used ECL detection, she does not see anything on her blot. Any suggestions as far as protocol etc.. would be most appreciated.

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Answer

Thank you for your enquiry. We would like to emphasize that ab1309 has not been tested on rat samples. It has been tested on mouse brain cell lysates as the Western blot image indicates, so we would suggest using this type of sample as positive control. Customer also should test the secondary antibody to make sure that it works properly. There is no information in your e-mail if it has been done or not. There is no information how the lysate was prepared. Any protease inhibitors? Is the target protein intact in the samples? How is PPP2CA expressed in rat liver and skeletal muscle? Is the expression of PPP2CA lower or higher compared to human or rat brain? Perhaps the customer should test different concentration of the primary antibody. If you need anything further or any help then please let us know. Please complete and forward our on-line Western blot Questionnaire.

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