Anti-PP2A alpha + beta 抗体 (ab1309)
製品の概要
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製品名
Anti-PP2A alpha + beta antibody
PP2A alpha + beta 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PP2A alpha + beta -
由来種
Goat -
特異性
This antibody will recognise both alpha and beta isoforms, which are almost identical. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Dog, Pig -
免疫原
Synthetic peptide corresponding to Human PP2A alpha + beta aa 297-309 (C terminal).
Sequence:C-EPHVTRRTPDYFL
(Peptide available asab22802) -
ポジティブ・コントロール
- WB: Recombinant Human PP2A-alpha protein (ab114424) can be used as a positive control; Mouse/Rat/Pig brain whole cell lysate in RIPA buffer.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab1309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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WB | Use a concentration of 0.03 - 3 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).Can be blocked with Human PP2A alpha + beta peptide (ab22802). |
ターゲット情報
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機能
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'. -
配列類似性
Belongs to the PPP phosphatase family. PP-1 subfamily. -
翻訳後修飾
Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
細胞内局在
Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2. - Information by UniProt
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参照データベース
- Entrez Gene: 5515 Human
- Entrez Gene: 5516 Human
- Entrez Gene: 19052 Mouse
- Entrez Gene: 19053 Mouse
- Entrez Gene: 397136 Pig
- Entrez Gene: 397656 Pig
- Entrez Gene: 24672 Rat
- Entrez Gene: 24673 Rat
see all -
別名
- PP2A A antibody
- PP2A alpha antibody
- PP2A B antibody
see all
画像
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All lanes : Anti-PP2A alpha + beta antibody (ab1309) at 0.03 µg/ml
Lane 1 : Mouse brain whole cell lysate in RIPA buffer
Lane 2 : Rat brain whole cell lysate in RIPA buffer
Lane 3 : Pig brain whole cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 36 kDaPrimary incubation was 1 hour.
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Anti-PP2A alpha + beta antibody (ab1309) at 0.05 µg/ml + Human Cerebellum lysates at 35 µg
Developed using the ECL technique.
Predicted band size: 36 kDaPrimary incubated for 1 hour.
プロトコール
データシートおよび資料
参考文献
ab1309 has not yet been referenced specifically in any publications.