Anti-PP2A-alpha 抗体 (ab106262)
Key features and details
- Rabbit polyclonal to PP2A-alpha
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PP2A-alpha antibody
PP2A-alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PP2A-alpha -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106262の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa.
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| IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml. Boiling the sections is required.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa. |
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Boiling the sections is required. |
ターゲット情報
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機能
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. -
配列類似性
Belongs to the PPP phosphatase family. PP-1 subfamily. -
翻訳後修飾
Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
細胞内局在
Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2. - Information by UniProt
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参照データベース
- Entrez Gene: 5515 Human
- Entrez Gene: 19052 Mouse
- Entrez Gene: 24672 Rat
- Omim: 176915 Human
- SwissProt: P67775 Human
- SwissProt: P63330 Mouse
- SwissProt: P63331 Rat
- Unigene: 105818 Human
see all -
別名
- PP2A alpha antibody
- PP2A-alpha antibody
- PP2AA_HUMAN antibody
see all
画像
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Western blot - Anti-PP2A-alpha antibody (ab106262)All lanes : Anti-PP2A-alpha antibody (ab106262) at 0.5 µg/ml
Lane 1 : Rat kidney tissue Lysate
Lane 2 : Rat Liver tissue Lysate
Lane 3 : Human Placenta tissue Lysate
Lane 4 : Mouse Kidney tissue Lysate
Lane 5 : A431 Whole Cell Lysate
Lane 6 : HeLa Whole Cell Lysate
Lane 7 : 293T Whole Cell Lysate
Lane 8 : NIH Whole Cell Lysate
Lane 9 : PC12 Whole Cell Lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 36 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A-alpha antibody (ab106262)Cytoplasmic staining of PP2A in a formalin fixed, paraffin embedded section of human mammary cancer, using ab106262 at a concentration of 2µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab106262 は 5 報の論文で使用されています。
- Mazhar S et al. Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac. Cell Rep 30:3164-3170.e3 (2020). PubMed: 32130915
- Leonard D et al. Selective PP2A Enhancement through Biased Heterotrimer Stabilization. Cell 181:688-701.e16 (2020). PubMed: 32315618
- Stafman LL et al. PP2A activation alone and in combination with cisplatin decreases cell growth and tumor formation in human HuH6 hepatoblastoma cells. PLoS One 14:e0214469 (2019). PubMed: 30969990
- Umesalma S et al. RABL6A inhibits tumor-suppressive PP2A/AKT signaling to drive pancreatic neuroendocrine tumor growth. J Clin Invest 130:N/A (2019). PubMed: 30721156
- Yi H et al. Isosteviol Protects Free Fatty Acid- and High Fat Diet-Induced Hepatic Injury via Modulating PKC-ß/p66Shc/ROS and Endoplasmic Reticulum Stress Pathways. Antioxid Redox Signal 30:1949-1968 (2019). PubMed: 30484323