製品の概要

  • 製品名

    PP 1, Src family tyrosine kinase inhibitor
  • 製品の詳細

    Potent Src family tyrosine kinase inhibitor
  • 生理活性の詳細

    Potent Src family tyrosine kinase inhibitor. Inhibits a multitude of kinases (IC50 values are 0.05 (LCK), 0.52 (CSK), 0.64 (p38α) and 1.06 (CK1δ) µM). Inhibits Bcr-Abl kinase and induces apoptosis.
  • 精製度

    > 98%
  • CAS 番号

    172889-26-8
  • 構造式

    Chemical Structure

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab120859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • Herpes simplex virus-2 regulates ER-alpha and HA via Src kinase in ECT-1 cells.

    (Panel B) ECT-1 cells were treated with vehicle (DMSO) or Src inhibitor (SKI/PP1). ER-alpha was quantified using densitometry following Western blot analysis (ANOVA with Tukey’s multiple comparison; NT vs VEH, n.s.; NT vs SKI/PPI, p< .02; VEH vs SKI/PPI, p< .01).

  • ab11512 staining E cadherin in SW480 cells treated with PP1 (ab120859), by ICC/IF. Increase of E cadherin expression correlates with increased concentration of PP1, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120859 (PP1) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

参考文献

This product has been referenced in:

  • Tatton L  et al. The Src-selective kinase inhibitor PP1 also inhibits Kit and Bcr-Abl tyrosine kinases. J Biol Chem 278:4847-53 (2003). Read more (PubMed: 12475982) »
  • Bain J  et al. The specificities of protein kinase inhibitors: an update. Biochem J 371:199-204 (2003). Read more (PubMed: 12534346) »
  • Hanke JH  et al. Discovery of a novel, potent, and Src family-selective tyrosine kinase inhibitor. Study of Lck- and FynT-dependent T cell activation. J Biol Chem 271:695-701 (1996). Read more (PubMed: 8557675) »
See 1 Publication for this product

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