Anti-PON1 抗体 [EPR2893] - BSA and Azide free (ab248121)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2893] to PON1 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PON1 antibody [EPR2893] - BSA and Azide free
PON1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2893] to PON1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IP: Human liver lysate.. WB: Human mouse and rat plasma and liver lysate
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特記事項
ab248121 is the carrier-free version of ab126597.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2893 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248121の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
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特記事項 |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
ターゲット情報
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機能
Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. -
組織特異性
Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. -
関連疾患
Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. -
配列類似性
Belongs to the paraoxonase family. -
翻訳後修飾
Glycosylated.
The signal sequence is not cleaved.
Present in two forms, form B contains a disulfide bond, form A does not. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
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参照データベース
- Entrez Gene: 5444 Human
- Entrez Gene: 18979 Mouse
- Entrez Gene: 84024 Rat
- Omim: 168820 Human
- SwissProt: P27169 Human
- SwissProt: P52430 Mouse
- SwissProt: P55159 Rat
- Unigene: 370995 Human
see all -
別名
- A esterase 1 antibody
- A-esterase 1 antibody
- Aromatic esterase 1 antibody
see all
画像
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This data was developed using ab226122, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Adiponectin with purified ab226122 at 1/50 dilution (1.88 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
All lanes : Anti-PON1 antibody [EPR2893] (ab126597) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human liver lysate
Lane 3 : Mouse plasma lysate
Lane 4 : Mouse liver lysate
Lane 5 : Rat plasma lysate
Lane 6 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40-45 kDa why is the actual band size different from the predicted?This data was developed using ab126597, the same antibody clone in a different buffer formulation.
The expression profile and molecular weights observed are consistent with what have been described in the literatures (PMID: 17906223, 30262871).
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This data was developed using ab126597, the same antibody clone in a different buffer formulation.
Purified ab126597 at 1/20 dilution (0.5µg) immunoprecipitating PON1 in Human liver lysate.
Lane 1 (input): Human liver lysate 10µg
Lane 2 (+): ab126597 + Human liver lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126597 in Human liver lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 40-45 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248121 は論文での使用が確認できていません。