Anti-POMK 抗体 (ab272564)
Key features and details
- Rabbit polyclonal to POMK
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-POMK antibody -
製品の詳細
Rabbit polyclonal to POMK -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
免疫原
Recombinant fragment corresponding to Human POMK aa 200-350.
Database link: Q9H5K3 -
ポジティブ･コントロール
- WB: HEK-293T cells. IHC-P: Human placenta tissue. ICC/IF: U-251 MG.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif. -
関連疾患
Muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies A12 -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. STKL subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 84197 Human
- Omim: 615247 Human
- SwissProt: Q9H5K3 Human
- Unigene: 491646 Human
-
別名
- FLJ23356 antibody
- MDDGA12 antibody
- POMK antibody
see all
画像
-
Western blot - Anti-POMK antibody (ab272564)Lanes 2-3 : Anti-POMK antibody (ab272564) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : Control (vector only transfected HEK-293T lysate)
Lane 3 : POMK over-expression lysate (mammalian HEK-293T cells) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POMK antibody (ab272564)Paraffin-embedded formalin-fixed Human placenta tissue stained for POMK using ab272564 at 1/50 in immunohistochemical analysis.
-
Immunocytochemistry/ Immunofluorescence - Anti-POMK antibody (ab272564)PFA fixed, Triton X-100 permeablized human cell line U-251 MG labeling POMK (green) using ab272564 at 2 ug/ml in ICC/IF analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab272564 は論文での使用が確認できていません。