Anti-Polyethylene glycol 抗体 [EPR21993-154] (ab203857)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21993-154] to Polyethylene glycol
- Suitable for: Functional Studies, WB, ELISA, Sandwich ELISA
- Reacts with: Species independent
Related conjugates and formulations
製品の概要
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製品名
Anti-Polyethylene glycol antibody [EPR21993-154]
Polyethylene glycol 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR21993-154] to Polyethylene glycol -
由来種
Rabbit -
アプリケーション
適用あり: Functional Studies, WB, ELISA, Sandwich ELISAmore details
適用なし: IHC-P -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: BSA-methoxy PEG 5K lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR21993-154 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab203857の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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WB |
1/1000.
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ELISA |
1/100.
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Sandwich ELISA |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
WB
1/1000. |
ELISA
1/100. |
Sandwich ELISA
Use at an assay dependent concentration. |
ターゲット情報
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別名
- PEG antibody
画像
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Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857) at 1/1000 dilution + BSA-methoxy PEG 5K, 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 250 kDa why is the actual band size different from the predicted?Exposure time: 92 seconds.
Blocking/Diluting buffer: 5% NFDM/TBST.
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Direct ELISA antigen dose-response curve using AffniPure Goat Anti-Mouse IgG (H&L) at 1 µg/ml concentration. The detector antibody was ab203857 anti-Polyethylene glycol at 1 µg/ml. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.
Antigen concentration range: 0-1000 ng/ml.
Blocking/Diluting Buffer and concentration: 1% BSA/TBS.
Washing Buffer: 1 X TBST.
Substrate: p-nitrophenyl phosphate (PNPP).
Coloration time: 10 min.
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Direct ELISA antigen dose-response curve using ab203857 at 2 µg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.
Antigen concentration range: 0-1000 ng/ml.
Blocking/Diluting Buffer and concentration: 1% BSA/TBS.
Washing Buffer: 1 X TBST.
Substrate: 3,3',5,5'-Tetramethylbenzidine (TMB).
Coloration time: 2 min.
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Direct ELISA antigen dose-response curve using ab203857 at 2 µg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.
Antigen concentration range: 0-1000 ng/ml.
Blocking/Diluting Buffer and concentration: 1% BSA/TBS.
Washing Buffer: 1 X TBST.
Substrate: 3,3',5,5'-Tetramethylbenzidine (TMB).
Coloration time: 2 min.
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Direct ELISA antigen dose-response curve using ab203857 at 0.05 µg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.
Antigen concentration range: 0-0.05µg/ml.
Blocking/Diluting Buffer and concentration: 1% BSA/TBS.
Washing Buffer: 1 X TBST.
Substrate: p-nitrophenyl phosphate (PNPP).
Coloration time: 15 min.
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Direct ELISA antigen dose-response curve using ab203857 at 0.05 µg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.
Antigen concentration range: 0-0.05µg/ml.
Blocking/Diluting Buffer and concentration: 1% BSA/TBS.
Washing Buffer: 1 X TBST.
Substrate: p-nitrophenyl phosphate (PNPP).
Coloration time: 15 min.
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Method: APS sensor+ Antigen (methoxy PEG800 at 0.625 µg/ml) + Blocking (BSA) + Antibody (ab203857 at 2, 1, 0.5, 0.25, 0.125µg/ml).
Results:
KD (M) = 5.81E-11
kon(1/Ms) = 2.70E+05
kon Error = 6.28E+02
kdis(1/s) = 1.57E-05
kdis Error = 4.49E-07
Full X2= 10.143
Full R2= 0.9993
Testing Biosensor: Aminopropylsilane (APS) Biosensors
Testing machine: Fortebio RED96e
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Method: APS sensor+ Antigen (methoxy PEG800 at 25 µg/ml) + Blocking (BSA) + Antibody (ab203857 at 0.5, 0.25, 0.125, 0.0625, 0.03125µg/ml).
Results:
KD (M) = 1.29E-10
kon(1/Ms) = 2.23E+06
kon Error = 1.45E+04
kdis(1/s) = 2.88E-04
kdis Error = 1.74E-06
Full X2 = 3.475
Full R2 = 0.9865
Testing Biosensor: Aminopropylsilane (APS) Biosensors
Testing machine: Fortebio RED96e
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab203857 は論文での使用が確認できていません。