Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6.0) for 10 min at 95°C
Permeabilization
No
Specification
Liver cancer
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% goat serum in PBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: goat anti-mouse biotinylated secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Primary antibody was omitted for the negative control.
Antigen retrieval was performed in citrate buffer (pH 6.0) for 10 min at 95°C
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投稿 Jun 08 2021