Application
Western blot
Sample
Human Cell lysate - other (HEK293T)
Gel Running Conditions
Non-reduced Denaturing (12%SDS)
Loading amount
20 µg
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% MILK
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Amersham ECL HRP Conjugated Antibodies
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ecl
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 150 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. manohar kodavati
Verified customer
投稿 Jan 03 2020