Anti-POLR2E/rpb5 抗体 [EPR11750] (ab180151)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11750] to POLR2E/rpb5
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-POLR2E/rpb5 antibody [EPR11750]
POLR2E/rpb5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11750] to POLR2E/rpb5 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human POLR2E/rpb5 aa 1-100. The exact sequence is proprietary.
Database link: P19388 -
ポジティブ・コントロール
- HepG2, A431 and HeLa whole cell lysate (ab150035); Human kidney tissue; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR11750 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180151の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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WB |
1/5000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
|
特記事項 |
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Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/5000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
ターゲット情報
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機能
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. -
配列類似性
Belongs to the archaeal rpoH/eukaryotic RPB5 RNA polymerase subunit family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5434 Human
- Entrez Gene: 66420 Mouse
- Entrez Gene: 690966 Rat
- Omim: 180664 Human
- SwissProt: P19388 Human
- SwissProt: Q80UW8 Mouse
- SwissProt: B0BNE2 Rat
- Unigene: 24301 Human
see all -
別名
- and III subunit ABC1 antibody
- and III subunit RPABC1 antibody
- DNA directed RNA polymerase II 23 kda polypeptide antibody
see all
画像
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All lanes : Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151) at 1/20000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 25 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling POLR2E/rpb5 with ab180151 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling POLR2E/rpb5 with ab180151 at 1/10 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green).
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Anti-POLR2E/rpb5 antibody [EPR11750] (ab180151) at 1/5000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 25 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab180151 は 1 報の論文で使用されています。
- Lee YL et al. MAF1 represses CDKN1A through a Pol III-dependent mechanism. Elife 4:e06283 (2015). ChIP ; Human . PubMed: 26067234