リコンビナント
RabMAb

Anti-Poliovirus Receptor 抗体 [EPR17302] - BSA and Azide free (ab228348)

製品の概要

  • 製品名
    Anti-Poliovirus Receptor antibody [EPR17302] - BSA and Azide free
    Poliovirus Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR17302] to Poliovirus Receptor - BSA and Azide free
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment within Human Poliovirus Receptor aa 200-350. The exact sequence is proprietary.
    Database link: P15151

  • 特記事項

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab228348 is a PBS-only buffer format of ab205304. Please refer to ab205304 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR17302
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab228348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa).
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.
  • 配列類似性
    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD155 antibody
    • CD155 antigen antibody
    • FLJ25946 antibody
    • HVED antibody
    • mE4 antibody
    • NECL 5 antibody
    • Necl-5 antibody
    • Necl5 antibody
    • Nectin like 5 antibody
    • Nectin like protein 5 antibody
    • Nectin-like protein 5 antibody
    • Ortholog of mouse Tage4 antibody
    • Poliovirus receptor antibody
    • PVR antibody
    • PVR_HUMAN antibody
    • PVS antibody
    • Taa1 antibody
    • Tage 4 antibody
    • TAGE4 antibody
    see all

画像

  • Poliovirus Receptor was immunoprecipitated from 1mg of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with ab205304 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205304 at 1/2000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
    Lane 1: U-87 MG whole cell lysate 10µg (Input).
    Lane 2: ab205304 IP in U-87 MG whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205304 in U-87 MG whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205304).

参考文献

ab228348 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab228348.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録