製品の概要

  • 製品名
    Anti-Poliovirus Receptor antibody [EPR17302]
    Poliovirus Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR17302] to Poliovirus Receptor
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment within Human Poliovirus Receptor aa 200-350. The exact sequence is proprietary.
    Database link: P15151

  • ポジティブ・コントロール
    • WB: HT1080, U-87 MG, HUVEC and K562 whole cell lysates; human fetal heart, fetal kidney and fetal spleen lysates. IP: U-87 MG whole cell lysate.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR17302
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab205304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa).
IP 1/30.

ターゲット情報

  • 機能
    Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.
  • 配列類似性
    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD155 antibody
    • CD155 antigen antibody
    • FLJ25946 antibody
    • HVED antibody
    • mE4 antibody
    • NECL 5 antibody
    • Necl-5 antibody
    • Necl5 antibody
    • Nectin like 5 antibody
    • Nectin like protein 5 antibody
    • Nectin-like protein 5 antibody
    • Ortholog of mouse Tage4 antibody
    • Poliovirus receptor antibody
    • PVR antibody
    • PVR_HUMAN antibody
    • PVS antibody
    • Taa1 antibody
    • Tage 4 antibody
    • TAGE4 antibody
    see all

画像

  • All lanes : Anti-Poliovirus Receptor antibody [EPR17302] (ab205304) at 1/4000 dilution

    Lane 1 : HT1080 (Human fibrosarcoma cell line) whole cell lysate
    Lane 2 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Poliovirus Receptor antibody [EPR17302] (ab205304) at 1/1000 dilution + K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Poliovirus Receptor antibody [EPR17302] (ab205304) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Poliovirus Receptor antibody [EPR17302] (ab205304) at 1/1000 dilution

    Lane 1 : Untreated K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate deglycosylation (PNGase F) treated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Poliovirus Receptor was immunoprecipitated from 1mg of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with ab205304 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205304 at 1/2000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
    Lane 1: U-87 MG whole cell lysate 10µg (Input).
    Lane 2: ab205304 IP in U-87 MG whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205304 in U-87 MG whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

参考文献

ab205304 has not yet been referenced specifically in any publications.

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