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Anti-Podoplanin / gp36 抗体 [PMab-1] - BSA and Azide free (ab256564)

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Western blot - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
  • Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
  • Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
  • Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [PMab-1] to Podoplanin / gp36 - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P, WB
  • Reacts with: Mouse

製品の概要

  • 製品名

    Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free
    Podoplanin / gp36 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [PMab-1] to Podoplanin / gp36 - BSA and Azide free

  • 由来種

    Rat

  • アプリケーション

    適用あり: IHC-Fr, IHC-P, WBmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse lung tissue lysate. IHC-P: Mouse kidney and lung tissue. IHC-Fr: Mouse kidney and lung tissue.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab256564 is the carrier-free version of ab256559. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab256564の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
1/500.
IHC-P
1/1000.
WB
1/1000. Predicted molecular weight: 16 kDa.
特記事項
IHC-Fr
1/500.
IHC-P
1/1000.
WB
1/1000. Predicted molecular weight: 16 kDa.
  • 追加情報
    Is unsuitable for ICC/IF.

    • ターゲット情報

    • 機能

      May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.

    • 組織特異性

      Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.

    • 配列類似性

      Belongs to the podoplanin family.

    • 翻訳後修飾

      Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
      The N-terminus is blocked.

    • 細胞内局在

      Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Aggrus antibody
        • Glycoprotein 36 KD antibody
        • Glycoprotein 36 antibody
        • gp 36 antibody
        • GP 38 antibody
        • GP 40 antibody
        • gp36 antibody
        • GP38 antibody
        • GP40 antibody
        • HT1A 1 antibody
        • HT1A1 antibody
        • hT1alpha 1 antibody
        • hT1alpha 2 antibody
        • hT1alpha1 antibody
        • hT1alpha2 antibody
        • Lung type I cell membrane associated glycoprotein antibody
        • Lung type I cell membrane associated glycoprotein isoform a antibody
        • Lung type I cell membrane associated glycoprotein T1A 2 antibody
        • OTS 8 antibody
        • OTS8 antibody
        • OTTHUMP00000009640 antibody
        • OTTHUMP00000044504 antibody
        • PA2.26 antibody
        • PA2.26 antigen antibody
        • Pdpn antibody
        • PDPN_HUMAN antibody
        • Podoplanin antibody
        • PSEC0003 antibody
        • PSEC0025 antibody
        • T1 alpha antibody
        • T1 ALPHA GENE antibody
        • T1-alpha antibody
        • T1A 2 antibody
        • T1A antibody
        • TI1A antibody
        • TIA 2 antibody
        • TIA2 antibody
        see all

      画像

      • Western blot - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
        Western blot - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
        Anti-Podoplanin / gp36 antibody [PMab-1] (ab256559) at 1/1000 dilution + mouse lung tissue lysate at 20 µg

        Secondary
        Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

        Predicted band size: 16 kDa



        Blocking/Dilution buffer: 5% NFDM/TBST.

        Exposure time 26 secs.

        This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)

        Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Podoplanin / gp36 with ab256559 at 1/1000 dilution followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse lung is observed. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

        Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)

        Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Podoplanin / gp36 with ab256559 at 1/1000 dilution followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on the podocytes in the mouse kidney is observed. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

        Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

      • Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
        Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)

        Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Podoplanin / gp36 with ab256559 at 1/500 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse lung is observed. The nuclear counterstain was DAPI (Blue).

        Secondary antibody control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

        Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

        This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

      • Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
        Immunohistochemistry (Frozen sections) - Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)

        Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Podoplanin / gp36 with ab256559 at 1/500 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000  dilution (Green). Positive staining on mouse kidney is observed. The nuclear counterstain was DAPI (Blue).

        Secondary antibody control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

        Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

        This image was produced using the same antibody clone but in a different formulation ab256559, PBS, sodium azide, glycerol and BSA.

      • Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)
        Anti-Podoplanin / gp36 antibody [PMab-1] - BSA and Azide free (ab256564)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (4)

      ab256564 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab256564 は 4 報の論文で使用されています。

      • Yamada S  et al. Epitope mapping of anti-mouse podoplanin monoclonal antibody PMab-1. Biochem Biophys Rep 15:52-56 (2018). PubMed: 29998193
      • Yamada S  et al. Development of mPMab-1, a Mouse-Rat Chimeric Antibody Against Mouse Podoplanin. Monoclon Antib Immunodiagn Immunother 36:77-79 (2017). PubMed: 28387612
      • Maruyama Y  et al. The effect of podoplanin inhibition on lymphangiogenesis under pathological conditions. Invest Ophthalmol Vis Sci 55:4813-22 (2014). PubMed: 24985477
      • Kaji C  et al. Immunohistochemical Examination of Novel Rat Monoclonal Antibodies against Mouse and Human Podoplanin. Acta Histochem Cytochem 45:227-37 (2012). PubMed: 23012488

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