Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (rat alveolar sections)
Specification
rat alveolar sections
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% saponin
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 10% serum, 0.2% saponin in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor® 488 Goat Anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/300
Additional data
Additional Notes
We do see very strong signals in tissue sections as well as in isolated cells from rat lungs. We suspect that the epitope for this antibody is located in the C-terminus of podoplanin which is highly homologous between human and rat (aa129-155)
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投稿 Nov 08 2012