Anti-Podoplanin / gp36 抗体 (ab10274)
Key features and details
- Rabbit polyclonal to Podoplanin / gp36
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Podoplanin / gp36 antibody
Podoplanin / gp36 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Podoplanin / gp36 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment (His-tag) corresponding to Human Podoplanin/ gp36 aa 1-150.
Database link: Q86YL7 -
ポジティブ･コントロール
- WB: LEC whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:The lyophilized IgG is stable at 4°C for at least one month and for more than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six months at 2-4°C in the presence of a preservative. Reconstituted antibody can also be aliquoted and stored at –20°C to –70°C for at least 1 year without detectable loss of activity. Avoid repeated freeze-thaw cycles. -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10274の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa. |
ターゲット情報
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機能
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. -
組織特異性
Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. -
配列類似性
Belongs to the podoplanin family. -
翻訳後修飾
Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
The N-terminus is blocked. -
細胞内局在
Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. - Information by UniProt
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参照データベース
- Entrez Gene: 10630 Human
- Omim: 608863 Human
- SwissProt: Q86YL7 Human
- Unigene: 468675 Human
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別名
- Aggrus antibody
- Glycoprotein 36 KD antibody
- Glycoprotein 36 antibody
see all
画像
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Western blot - Anti-Podoplanin / gp36 antibody (ab10274)
Western Blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC). The total lysate was subjected to SDS-PAGE and subsequent Western analysis with the polyclonal antibody ab10274.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab10274 は 4 報の論文で使用されています。
- Kwak J et al. Isolation and characterization of tumorspheres from a recurrent pineoblastoma patient: Feasibility of a patient-derived xenograft. Int J Oncol 49:569-78 (2016). PubMed: 27277549
- Carden CP et al. The association of PI3 kinase signaling and chemoresistance in advanced ovarian cancer. Mol Cancer Ther 11:1609-17 (2012). PubMed: 22556379
- Kong BH et al. Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens. Childs Nerv Syst : (2012). PubMed: 23143002
- Link A et al. Association of T-zone reticular networks and conduits with ectopic lymphoid tissues in mice and humans. Am J Pathol 178:1662-75 (2011). PubMed: 21435450
