Application
IHC - Wholemount
Sample
Rat Embryo (E13.5 Rat Embryo)
Specification
E13.5 Rat Embryo
Other product details
Incubation time
99 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS 1x +0.5% Gelatin +0.5% Triton
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Abcam antibody:Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed
Additional data
Additional Notes
4%PFA immersion-fixed (1 day)
Incubated for 12 days at 37°C in primary antibody
then cleared using iDisco+ protocol
Imaged with light sheet microscopy
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alain Chedotal
Verified customer
投稿 Jan 31 2022