Application
Western blot
Sample
Human Cell lysate - whole cell (SW1353 human chondrosacroma cell line)
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris Gel)
Loading amount
30 µg
Specification
SW1353 human chondrosacroma cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in 5% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-mouse IgG HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
NA
Exposure
10 second(s)
Bands
Specific: 30 kDa
Positive control
non-treated SW1353 human chondrosacroma cell line
Additional data
Additional Notes
With the use of the current Abcam antibody, we demonstrated for the first time human chondrosacroma SW1353 cells express podoplanin. Current antibody can also gives a single clear band in WB with total cell lysate from SW1353 cells using reducing and denatured conditions.
The current finding suggested an important future application of anti-podoplanin in chondrosacroma related research.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 15 2014