Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5614] to PMP70 - BSA and Azide free
- Suitable for: ICC, Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
製品名Anti-PMP70 antibody [EPR5614] - BSA and Azide free
PMP70 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5614] to PMP70 - BSA and Azide free
アプリケーション適用あり: ICC, Flow Cyt, WBmore details
適用なし: IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human PMP70 aa 600-700 (C terminal). The exact sequence is proprietary.
- WB: HeLa and HAP1 cell lysates.
Ab247868 is the carrier-free version of ab109448. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247868 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 4.10 x 10 -11 M Learn more about KD
Concentration information loading...
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab247868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 75 kDa).|
機能Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity.
配列類似性Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain.
- Information by UniProt
- 70 kDa peroxisomal membrane protein antibody
- ABC 43 antibody
- ABC D3 antibody
All lanes : Anti-PMP70 antibody [EPR5614] (ab109448) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PMP70 knockout HeLa cell lysate
Lane 3 : Wild-type HAP1 cell lysate
Lane 4 : PMP70 knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 75 kDa
This data was developed using the same antibody clone in a different buffer formulation (ab109448).
ab109448 Anti-PMP70 antibody [EPR5614] was shown to specifically react with PMP70 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265294 (knockout cell lysate ab257134) was used. Wild-type and PMP70 knockout samples were subjected to SDS-PAGE. ab109448 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.