Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk in TBST
Dilution
1/1000
Detection
Detection method
ECL+
Negative control
Lanes 1 and 2 contained fibroblast lysate: no band observed
Land 4 contained LCL lysate: no band observed
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: <70 kDa
Positive control
Lane 3 contained HEK293T lysate: specific band observed
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投稿 Apr 29 2019