Anti-PMP22 抗体 [EPR23112-110] (ab270400)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23112-110] to PMP22
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PMP22 antibody [EPR23112-110]
PMP22 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23112-110] to PMP22 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, IP, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human leg nerve tissue lysate and 293T transfected with PMP22 (WT) expression vector containing a myc-His-tag, whole cell lysate. IHC-P: Human nerve tissue. ICC/IF: 293T transfected with myc-his tagged PMP22 overexpression construct cells. Flow Cyt: 293T transfected with myc-his tagged PMP22 overexpression construct cells. IP: 293T transfected with myc-his tagged PMP22 overexpression construct cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23112-110 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab270400の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000. Predicted molecular weight: 18 kDa.
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IHC-P |
1/32000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250.
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IP |
1/30.
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Flow Cyt |
1/600.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 18 kDa. |
IHC-P
1/32000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250. |
IP
1/30. |
Flow Cyt
1/600. |
ターゲット情報
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機能
Might be involved in growth regulation, and in myelinization in the peripheral nervous system. -
関連疾患
Defects in PMP22 are the cause of Charcot-Marie-Tooth disease type 1A (CMT1A) [MIM:118220]; also known as hereditary motor and sensory neuropathy IA. CMT1A is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1A inheritance is autosomal dominant.
Defects in PMP22 are a cause of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
Defects in PMP22 are a cause of hereditary neuropathy with liability to pressure palsies (HNPP) [MIM:162500]; an autosomal dominant disorder characterized by transient episodes of decreased perception or peripheral nerve palsies after slight traction, compression or minor traumas.
Defects in PMP22 are the cause of Charcot-Marie-Tooth disease type 1E (CMT1E) [MIM:118300]; also known as Charcot-Marie-Tooth disease and deafness autosomal dominant. CMT1E is an autosomal dominant form of Charcot-Marie-Tooth disease characterized by the association of sensorineural hearing loss with peripheral demyelinating neuropathy.
Defects in PMP22 may be a cause of inflammatory demyelinating polyneuropathy (IDP) [MIM:139393]. IDP is a putative autoimmune disorder presenting in an acute (AIDP) or chronic form (CIDP). The acute form is also known as Guillain-Barre syndrome. -
配列類似性
Belongs to the PMP-22/EMP/MP20 family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5376 Human
- Omim: 601097 Human
- SwissProt: Q01453 Human
- Unigene: 372031 Human
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別名
- CMT1A antibody
- CMT1E antibody
- DSS antibody
see all
画像
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All lanes : Anti-PMP22 antibody [EPR23112-110] (ab270400) at 1/1000 dilution
Lane 1 : Human leg nerve tissue lysate
Lane 2 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 18,22 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsThe expression profile observed is consistent with what has been described in the literature (PMID: 25429154; 7649472).
Negative control: Human brain (PMID: 7649472).Blocking/diluting buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-PMP22 antibody [EPR23112-110] (ab270400) at 1/1000 dilution
Lane 1 : 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate
Lane 2 : 293T transfected with PMP22 (WT) expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 18,20 kDa why is the actual band size different from the predicted?
Exposure time: 15 seconds -
PMP22 was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) transfected with PMP22 (WT) expression vector containing a myc-His-tag®, whole cell lysate with ab270400 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270400 1/1000 dilution (0.58 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: 293T cells transfected with PMP22 (WT) expression vector containing a myc-His-tag®, whole cell lysate 10 μg
Lane 2: ab270400 IP in 293T transfected with PMP22 (WT) expression vector containing a myc-His-tag®, whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270400 in 293T transfected with PMP22 (WT) expression vector containing a myc-His-tag®, whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds
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Immunohistochemical analysis of paraffin-embedded Human nerve tissue labeling PMP22 with ab270400 at 1/32000 dilution (0.018 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in Schwann cells of human nerve (PMID:7691737) is observed. The section was incubated with ab270400 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PMP22 with ab270400 at 1/32000 dilution (0.018 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Negative control: negative staining in human kidney (PMID:7649472).
The section was incubated with ab270400 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells transfected with a myc-tagged PMP22 expression construct. cells labelling PMP22 with ab270400 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with a myc-tagged PMP22 expression construct is observed. 2233S Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized 293T transfected with myc-his tagged PMP22 overexpression construct cells labelling PMP22 with ab270400 at 1/600 dilution (0.1 ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with isotype control or ab270400. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab270400 は 1 報の論文で使用されています。
- Zhang J et al. Ulinastatin Promotes Regeneration of Peripheral Nerves After Sciatic Nerve Injury by Targeting let-7 microRNAs and Enhancing NGF Expression. Drug Des Devel Ther 14:2695-2705 (2020). PubMed: 32753848