Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8381] to Plunc - BSA and Azide free
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
製品名Anti-Plunc antibody [EPR8381] - BSA and Azide free
Plunc 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR8381] to Plunc - BSA and Azide free
アプリケーション適用あり: WB, Flow Cyt, IPmore details
適用なし: ICC or IHC-P
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human Plunc aa 150-250. The exact sequence is proprietary.
Ab248364 is the carrier-free version of ab131163. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248364 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 3.90 x 10 -12 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).|
|Flow Cyt||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
関連性May be involved in the airway inflammatory response after exposure to irritants. May be associated with tumor progression. May play a role in innate immune responses of the upper airways.
細胞内局在Secreted. Note: Found in the nasal mucus. Apical side of airway epithelial cells. Detected in nasal mucus
- BPIFA1 antibody
- bA49G10.5 antibody
- LPLUNC3 antibody
All lanes : Anti-Plunc antibody [EPR8381] (ab131163) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : PC3 cell lysate
Lane 3 : Human lung squamous cell cancer lysate
Lane 4 : Human fetal lung tissue lysate
Lane 5 : Jurkat cell lysate
Lane 6 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
This data was developed using ab131163, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.