製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to PLRG1
  • 由来種
    Goat
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human
  • 免疫原

    Synthetic peptide: PVSWKPEIIKRKRF, corresponding to C terminal amino acids 501-514 of Human PLRG1.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).Can be blocked with Human PLRG1 peptide (ab22860).
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

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  • Immunohistochemical analysis of paraffin embedded Human prostate tissue, staining PLRG1 with ab1372 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • All lanes : Anti-PLRG1 antibody (ab1372) at 0.1 µg/ml

    Lane 1 : HEK293 nuclear cell lysates
    Lane 2 : NIH3T3 nuclear cell lysates

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 57 kDa

  • Anti-PLRG1 antibody (ab1372) + Lysate prepared from mouse kidney extract at 35 µg

    Predicted band size: 57 kDa



    PLRG1 staining (0.5µg/ml) of Mouse Kidney extract (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

     

    Primary incubated for 1 hour.

プロトコール

参考文献

ab1372 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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