Application
Western blot
Sample
Human Tissue lysate - whole
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Pierce
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 20 may be kDa Non-specific: 30, 32, 50, 15, 10 kDa
Positive control
rat kidney extract
Additional data
Abcam response
The antibody did not work as described on the datasheet for this customer. We sent the customer a replacement vial and we are waiting to find out if the inconclusive result was due to a bad vial of antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 06 2006