Anti-PLK4 抗体 (ab2642)
Key features and details
- Goat polyclonal to PLK4
- Suitable for: ICC/IF, WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PLK4 antibody
PLK4 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PLK4 -
由来種
Goat -
アプリケーション
適用あり: ICC/IF, WB, Flow Cyt (Intra)more details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Dog -
免疫原
Synthetic peptide corresponding to Human PLK4 aa 1-100 (N terminal). (NP_001177728.1)
Database link: O00444 -
ポジティブ・コントロール
- Human colon lysate Flow Cyt (Intra): HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2642の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 109 kDa).
1 hour primary incubation is recommended for this product. |
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 109 kDa). 1 hour primary incubation is recommended for this product. |
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 1 POLO box domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Ubiquitinated; leading to its degradation by the proteasome.
Tyrosine-phosphorylated by TEC. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome > centriole. Nucleus > nucleolus. Cleavage furrow. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows. - Information by UniProt
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参照データベース
- Entrez Gene: 10733 Human
- Entrez Gene: 20873 Mouse
- SwissProt: O00444 Human
- SwissProt: Q64702 Mouse
- Unigene: 172052 Human
- Unigene: 3794 Mouse
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別名
- PLK 4 antibody
- PLK-4 antibody
- PLK4 antibody
see all
画像
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Immunofluorescence analysis of paraformaldehyde fixed HeLa cells staining PLK4. Cells were permeabilized with 0.15% Triton. Samples were incubated with primary anitbody for 1 hour at 10µg/ml. An Alexa Fluor 488 was used as the secondary antibody. DAPI was used as a nuclear counterstain. Unimmunized goat IgG (10µg/ml) was used as the negative control.
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Anti-PLK4 antibody (ab2642) at 2 µg/ml + human colon lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour -
Flow Cytometry analysis of HeLa cells labeling PLK4 with ab2642 at 10ug/mL followed by Alexa Fluor 488 secondary antibody (1ug/mL) (blue line). Cells were permeabilized with 0.5% Triton. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunofluorescence analysis of paraformaldehyde fixed A431 cells staining PLK4. Cells were permeabilized with 0.15% Triton. Samples were incubated with primary anitbody for 1 hour at 10µg/ml. An Alexa Fluor 488 was used as the secondary antibody. DAPI was used as a nuclear counterstain. Unimmunized goat IgG (10µg/ml) was used as the negative control.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab2642 は 10 報の論文で使用されています。
- Yang Y et al. CircKIF2A contributes to cell proliferation, migration, invasion and glycolysis in human neuroblastoma by regulating miR-129-5p/PLK4 axis. Mol Cell Biochem 476:2513-2525 (2021). PubMed: 33630225
- Yang XD et al. PLK4 deubiquitination by Spata2-CYLD suppresses NEK7-mediated NLRP3 inflammasome activation at the centrosome. EMBO J 39:e102201 (2020). PubMed: 31762063
- Xu Z et al. SNHG16 promotes tumorigenesis and cisplatin resistance by regulating miR-338-3p/PLK4 pathway in neuroblastoma cells. Cancer Cell Int 20:236 (2020). PubMed: 32536824
- Shi J et al. Long non-coding RNA RUNX1-IT1 plays a tumour-suppressive role in colorectal cancer by inhibiting cell proliferation and migration. Cell Biochem Funct 37:11-20 (2019). PubMed: 30499136
- Zhang Z et al. PLK4 is a determinant of temozolomide sensitivity through phosphorylation of IKBKE in glioblastoma. Cancer Lett 443:91-107 (2019). PubMed: 30529153
- Czub B et al. TOPORS, a Dual E3 Ubiquitin and Sumo1 Ligase, Interacts with 26 S Protease Regulatory Subunit 4, Encoded by the PSMC1 Gene. PLoS One 11:e0148678 (2016). PubMed: 26872363
- Rosario CO et al. A novel role for Plk4 in regulating cell spreading and motility. Oncogene N/A:N/A (2014). PubMed: 25174401
- Rosario CO et al. Plk4 is required for cytokinesis and maintenance of chromosomal stability. Proc Natl Acad Sci U S A 107:6888-93 (2010). PubMed: 20348415
- Martindill DM et al. Nucleolar release of Hand1 acts as a molecular switch to determine cell fate. Nat Cell Biol 9:1131-41 (2007). WB, ICC/IF ; Rat . PubMed: 17891141
- Syed N et al. Transcriptional silencing of Polo-like Kinase 2 (Snk/Plk2) is a frequent event in B cell malignancies. Blood : (2005). PubMed: 16160013