製品の概要
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製品名Anti-PLK4 antibody
PLK4 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to PLK4
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由来種Goat
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アプリケーション適用あり: ICC/IF, Flow Cyt, WBmore details
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種交差性交差種: Rat, Human
交差が予測される動物種: Mouse, Cow, Dog
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免疫原
Synthetic peptide:
ATCIGEKIEDFKVGN
, corresponding to N terminal amino acids 2-16 of Human PLK4. -
ポジティブ･コントロール
- Human colon lysate
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特記事項GenBank Accession Number - NP_055079
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab2642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| Flow Cyt | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 109 kDa).Can be blocked with Human PLK4 peptide (ab22924). |
ターゲット情報
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機能Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2.
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配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 1 POLO box domain.
Contains 1 protein kinase domain. -
翻訳後修飾Ubiquitinated; leading to its degradation by the proteasome.
Tyrosine-phosphorylated by TEC. -
細胞内局在Cytoplasm > cytoskeleton > centrosome > centriole. Nucleus > nucleolus. Cleavage furrow. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows.
- Information by UniProt
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参照データベース
- Entrez Gene: 10733 Human
- Entrez Gene: 20873 Mouse
- Entrez Gene: 310344 Rat
- SwissProt: O00444 Human
- SwissProt: Q64702 Mouse
- SwissProt: B2GUY1 Rat
- Unigene: 172052 Human
- Unigene: 3794 Mouse
see all -
別名
- PLK 4 antibody
- PLK-4 antibody
- PLK4 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-PLK4 antibody (ab2642)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells staining PLK4. Cells were permeabilized with 0.15% Triton. Samples were incubated with primary anitbody for 1 hour at 10µg/ml. An Alexa Fluor 488 was used as the secondary antibody. DAPI was used as a nuclear counterstain. Unimmunized goat IgG (10µg/ml) was used as the negative control. -
Immunocytochemistry/ Immunofluorescence - Anti-PLK4 antibody (ab2642)Immunofluorescence analysis of paraformaldehyde fixed A431 cells staining PLK4. Cells were permeabilized with 0.15% Triton. Samples were incubated with primary anitbody for 1 hour at 10µg/ml. An Alexa Fluor 488 was used as the secondary antibody. DAPI was used as a nuclear counterstain. Unimmunized goat IgG (10µg/ml) was used as the negative control. -
Western blot - Anti-PLK4 antibody (ab2642)Anti-PLK4 antibody (ab2642) at 2 µg/ml + human colon lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Observed band size: 110 kDa (why is the actual band size different from the predicted?)
Exposure time: 1 hour
参考文献
This product has been referenced in:
- Czub B et al. TOPORS, a Dual E3 Ubiquitin and Sumo1 Ligase, Interacts with 26 S Protease Regulatory Subunit 4, Encoded by the PSMC1 Gene. PLoS One 11:e0148678 (2016). Read more (PubMed: 26872363) »
- Rosario CO et al. A novel role for Plk4 in regulating cell spreading and motility. Oncogene N/A:N/A (2014). Read more (PubMed: 25174401) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"