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リコンビナントRabMAb

Anti-PLK1 (phospho T210) 抗体 [EPNCIR167] (ab155095)

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Western blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
  • Dot Blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
  • Western blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
  • Western blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
  • Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR167] to PLK1 (phospho T210)
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PLK1 (phospho T210) antibody [EPNCIR167]
    PLK1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPNCIR167] to PLK1 (phospho T210)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, Dot blotmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa lysate, treated with calyculin A; Human colon tissue and gastric carcinoma tissue.

  • 特記事項

    This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab155095の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 68 kDa.

For unpurified use at 1/1000 - 1/10000.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.
Dot blot
Use at an assay dependent concentration.
特記事項
WB
1/1000. Predicted molecular weight: 68 kDa.

For unpurified use at 1/1000 - 1/10000.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.
Dot blot
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.

  • 組織特異性

    Placenta and colon.

  • 配列類似性

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
    Contains 2 POLO box domains.
    Contains 1 protein kinase domain.

  • 発生段階

    Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.

  • 翻訳後修飾

    Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
    Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.

  • 細胞内局在

    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cell cycle regulated protein kinase antibody
      • PLK 1 antibody
      • PLK antibody
      • PLK-1 antibody
      • plk1 antibody
      • PLK1_HUMAN antibody
      • Polo like kinase 1 antibody
      • Polo-like kinase 1 antibody
      • Serine/threonine protein kinase 13 antibody
      • Serine/threonine protein kinase PLK1 antibody
      • Serine/threonine-protein kinase 13 antibody
      • Serine/threonine-protein kinase PLK1 antibody
      • STPK 13 antibody
      • STPK13 antibody
      see all

    画像

    • Western blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      Western blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      All lanes : Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) at 1/1000 dilution

      Lane 1 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysates with NFDM/TBST
      Lane 2 : Hela (Human cervix adenocarcinoma epithelial cell) treated with thymidine (2mM, 16 h) then with nocodazole (10nM, 24h). Whole cell lysates with NFDM/TBST
      Lane 3 : Hela (Human cervix adenocarcinoma epithelial cell) treated with thymidine (2mM, 16 h) then with nocodazole (10nM, 24h). Whole cell lysates.Then the membrane was incubated with phosphatase. with NFDM/TBST

      Lysates/proteins at 15 µg per lane.

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 68 kDa
      Observed band size: 68 kDa


      Exposure time: 10 seconds


      antibody used for ab155095 is purified batch

    • Dot Blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      Dot Blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

      Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) Dot Blot. Primary ab dilution 1:1000,  Secondary ab description and code (ab id)Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), Secondary ab dilution 1:100,000. Blocking buffer and concentration 5% NFDM/TBST, Diluting buffer and concentration 5% NFDM /TBST. Lane 1:PLK1 (pT210) phospho peptide, Lane 2: PLK1 non-phospho peptide, Exposure time 10 seconds. Note: antibody used for ab155095 is purified batch.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

      ab155095 staining PLK1 (phospho T210) in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An undiluted HRP-conjugated  anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    • Western blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      Western blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      All lanes : Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate post treatment with Nocodazole

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size: 68 kDa

    • Western blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      Western blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      All lanes : Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 68 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PLK1 with ab155095, unpurified, at 1/100 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

      Immunohistochemical analysis of paraffin-embedded Human gastroic carcinoma tissue labeling PLK1 with ab155095, unpurified, at 1/100 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

      Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab155095, unpurified, showing +ve staining.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

      Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155095, unpurified, showing +ve staining.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

      Immunohistochemical analysis of paraffin embedded Human placenta tissue using ab155095, unpurified, showing +ve staining.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)
      Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

    参考文献 (7)

    ab155095 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab155095 は 7 報の論文で使用されています。

    • Tanaka H  et al. YAP-dependent necrosis occurs in early stages of Alzheimer's disease and regulates mouse model pathology. Nat Commun 11:507 (2020). PubMed: 31980612
    • Liccardi G  et al. RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation. Mol Cell 73:413-428.e7 (2019). PubMed: 30598363
    • Ren Y  et al. PLK1 stabilizes a MYC-dependent kinase network in aggressive B cell lymphomas. J Clin Invest 128:5517-5530 (2018). PubMed: 30260324
    • Chen LL  et al. Phosphoproteome-based kinase activity profiling reveals the critical role of MAP2K2 and PLK1 in neuronal autophagy. Autophagy 13:1969-1980 (2017). PubMed: 28933595
    • Yamanishi E  et al. A novel form of necrosis, TRIAD, occurs in human Huntington's disease. Acta Neuropathol Commun 5:19 (2017). WB, IHC-P ; Human . PubMed: 28274274
    • Sur S  et al. Increased Expression of Phosphorylated Polo-Like Kinase 1 and Histone in Bypass Vein Graft and Coronary Arteries following Angioplasty. PLoS One 11:e0147937 (2016). IF . PubMed: 26820885
    • Mao Y  et al. Targeting TEAD/YAP-transcription-dependent necrosis, TRIAD, ameliorates Huntington's disease pathology. Hum Mol Genet 25:4749-4770 (2016). PubMed: 28171658

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