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リコンビナントRabMAb

Anti-Placental alkaline phosphatase (PLAP) 抗体 [SP15] (ab16695)

Submit a review Q&A (2)References (10)
Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
  • Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP15] to Placental alkaline phosphatase (PLAP)
  • Suitable for: Flow Cyt (Intra), IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

製品の概要

  • 製品名

    Anti-Placental alkaline phosphatase (PLAP) antibody [SP15]
    Placental alkaline phosphatase (PLAP) 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP15] to Placental alkaline phosphatase (PLAP)

  • 由来種

    Rabbit

  • 特異性

    This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-Pmore details
    適用なし: WB

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is considered to be commercially sensitive.

  • エピトープ

    Not determined

  • ポジティブ･コントロール

    • IHC-P: Seminoma and placenta tissues. Flow Cyt (intra): HeLa cells

  • 特記事項

    This product was switched from a hybridoma to recombinant production method on 7th March 2019.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 一次抗体 備考

    This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

  • ポリ/モノ

    モノクローナル

  • クローン名

    SP15

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab16695の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/200.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
特記事項
Flow Cyt (Intra)
1/200.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
追加情報
Is unsuitable for WB.

ターゲット情報

  • 配列類似性

    Belongs to the alkaline phosphatase family.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Alkaline phosphatase antibody
      • Alkaline phosphatase placental antibody
      • Alkaline phosphatase placental type antibody
      • Alkaline phosphatase Regan isozyme antibody
      • ALP antibody
      • Alp1 antibody
      • ALPP antibody
      • FLJ61142 antibody
      • Germ-cell alkaline phosphatase antibody
      • nagao isozyme antibody
      • OTTHUMP00000164354 antibody
      • PALP antibody
      • Placental alkaline phosphatase 1 antibody
      • placental heat-stable alkaline phosphatase antibody
      • placental type antibody
      • PLAP antibody
      • PLAP-1 antibody
      • PLAP1 antibody
      • PPB1_HUMAN antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
      Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

      Intracellular flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Placental alkaline phosphatase (PLAP) with purified ab16695 at 1/200 dilution (0.6µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. 

       This image was generated from the hybridoma version. 

        

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

      Human seminoma stained with ab16695.

      This image was generated from the hybridoma version.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

      Immunohistochemical analysis of paraffin-embedded human seminoma tissue labeling Alkaline Phosphatase with ab137879 at 1/50 dilution.

      This image was generated from the hybridoma version.

       

    • Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
      Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

    参考文献 (10)

    ab16695 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab16695 は 10 報の論文で使用されています。

    • Zhou Y  et al. The miR-204-5p/FOXC1/GDF7 axis regulates the osteogenic differentiation of human adipose-derived stem cells via the AKT and p38 signalling pathways. Stem Cell Res Ther 12:64 (2021). PubMed: 33461605
    • Cardot-Ruffino V  et al. Generation of an Fsp1 (fibroblast-specific protein 1)-Flpo transgenic mouse strain. Genesis 58:e23359 (2020). PubMed: 32191380
    • Ashley J  et al. Transsynaptic interactions between IgSF proteins DIP-a and Dpr10 are required for motor neuron targeting specificity. Elife 8:N/A (2019). PubMed: 30714906
    • Bracey DN  et al. Investigating the Osteoinductive Potential of a Decellularized Xenograft Bone Substitute. Cells Tissues Organs 207:97-113 (2019). PubMed: 31655811
    • Pang HB  et al. Virion stiffness regulates immature HIV-1 entry. Retrovirology 10:4 (2013). PubMed: 23305456
    • Saffarini CM  et al. Induction and persistence of abnormal testicular germ cells following gestational exposure to di-(n-butyl) phthalate in p53-null mice. J Androl 33:505-13 (2012). IHC-P ; Mouse . PubMed: 21868749
    • Schmid F  et al. Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) Degradation by Alkaline Phosphatase. J Biol Chem 287:32525-34 (2012). WB . PubMed: 22851169
    • Lu D  et al. Comparison of candidate serologic markers for type I and type II ovarian cancer. Gynecol Oncol 122:560-6 (2011). PubMed: 21704359
    • Laviolette LA  et al. 17beta-estradiol accelerates tumor onset and decreases survival in a transgenic mouse model of ovarian cancer. Endocrinology 151:929-38 (2010). IHC-P ; Mouse . PubMed: 20056833
    • Taylor DD  et al. Pregnancy-associated exosomes and their modulation of T cell signaling. J Immunol 176:1534-42 (2006). WB ; Human . PubMed: 16424182

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    We are interested in your products: “ab16695”, “ab16517” and “ab70988” but we need to know more about the sequence targeted by it. We would like to have as much information as possible about this question.

    Read More
    Answer

    Thank you for your enquiry.

    Here are the answers to your questions:

    1. ab16517 - immunogen is Full length native protein (purified) (Human) and epitope has not been mapped
    2. ab16695 - immunogen is Full length native protein (purified) (Human) and epitope has not been mapped
    3. ab70988 - peptide between 39˜69 amino acids from the N-terminal region of human BMPR1A

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Question

    Is there a western blot image available for this antibody?

    Read More
    Answer

    Please see the western blot in following reference noted under the 'References' section of the online datasheet for ab16695: Taylor DD et al. Pregnancy-associated exosomes and their modulation of T cell signaling. J Immunol 176:1534-42 (2006). WB; Human. PubMed: 16424182 I hope this is helpful. Please contact us again if you have any further questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com