Anti-Placental alkaline phosphatase (PLAP) 抗体 [8B6] (ab33)

Submit an Abreview Q&A (8)Specific References (4)

製品の概要

  • 製品名
    Anti-Placental alkaline phosphatase (PLAP) antibody [8B6]
    Placental alkaline phosphatase (PLAP) 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8B6] to Placental alkaline phosphatase (PLAP)
  • 由来種
    Mouse
  • 特異性
    Human placental alkaline phosphatase, Regan and Nagao isoenzymes. No cross reaction with other isoenzymes of AP.
  • アプリケーション
    適用あり: ELISA, IHC-P, RIA, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    HEp-2 cells with boosted surface expression of PLAP

  • ポジティブ･コントロール
    • Placenta.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    8B6
  • ミエローマ
    P3-NS1/1-Ag4-1
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-P 1/250 - 1/500.
RIA Use at an assay dependent concentration.
IHC-Fr 1/250 - 1/500.

ターゲット情報

  • 配列類似性
    Belongs to the alkaline phosphatase family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Alkaline phosphatase antibody
      • Alkaline phosphatase placental antibody
      • Alkaline phosphatase placental type antibody
      • Alkaline phosphatase Regan isozyme antibody
      • ALP antibody
      • Alp1 antibody
      • ALPP antibody
      • FLJ61142 antibody
      • Germ-cell alkaline phosphatase antibody
      • nagao isozyme antibody
      • OTTHUMP00000164354 antibody
      • PALP antibody
      • Placental alkaline phosphatase 1 antibody
      • placental heat-stable alkaline phosphatase antibody
      • placental type antibody
      • PLAP antibody
      • PLAP-1 antibody
      • PLAP1 antibody
      • PPB1_HUMAN antibody
      see all

    プロトコール

    参考文献

    This product has been referenced in:
    • Sharp TM  et al. Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal. PLoS One 5:e13130 (2010). ICC/IF ; Human . Read more (PubMed: 20976190) »
    • Zhang L  et al. Alteration in the IL-2 signal peptide affects secretion of proteins in vitro and in vivo. J Gene Med 7:354-65 (2005). ELISA ; Human . Read more (PubMed: 15619290) »

    See all 4 Publications for this product

    ab33 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    8 reviews or Q&A

    10
    Votes: Highest First

    Our lab is considering the antibody "Anti-placental alkaline phosphatase" with catalog #ab33.   The datasheet of this antibody indicates that the cross reactivity is "reacts with Human". We want to know if it can also reacts with mouse. I want ...

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    Thank you for your enquiry. Unfortunately we have not tested ab33 against mouse so we do not know whether it is suitable. The antibody was raised against the whole protein, and although the mouse and human protein show 75% homology we do not know wh...

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    I have a customer enquiry regarding ab33 (PLAP protein). She would like to know the expected molecular weight of the protein, she also wants to test the antibody for western blot. Although it is not on the datasheet as a tested application, she was ...

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    Thank you for your enquiry. According to the database SwissProt the MW of the precursor PLAP is 57kDa and it is glycosylated therefore its weight should be higher than this. A paper by Webb et al (Placenta. 1986 Sep-Oct;7(5):405-15.Isolation of pl...

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    About a year ago someone asked if the PLAP 8B6-ab33 antibody could be used in westernblotting. At that time you said it hasn't been tested for that yet. Has it been tested since, and can it be used in westernblotting? Mark

    I'm afraid that we do not have any data regarding the suitablity of this antibody for WB.

    Is this antibody suitable for flow cytometry?

    This antibody has not been tested in flow cytometry.

    Marseille 1 october 2001 dear sir, ab33 is an anti human alkaline phosphatase, I would like to know if this antibody is suitable for immunoflorescence in cell cultures. Thank you very much John-Baptist

    This antibody has not been tested in immunofluorescence.

    Hi, can you please tell me what epitope ab33 was raised against, please? I am trying to detect a fusion protein which has the AP sequence from nucleotides 32 to 1708. If the answer is proprietary, please let me know if it will detect a protein within...

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    This monoclonal was raised against the whole protein, and in the absence of any epitope mapping studies, I am afraid that we do not know against which part of the protein the antibody is reactive.

    Could you let me know whether antibodies against alkaline phospatase from human placenta inhibitor not the activity of alkaline phosphatase itself in solution or on the surface of the membrane? Thank you. Vlad

    Vlad We do not have any data on this antibody's inhibiting activity in vivo or in vitro. We are about to implement a trial 'try risk free' scheme which says that if an antibody is purchased and used in an experiment outside that written on the datashe...

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    I was wondering if this antibody (alkaline phosphatase 8B6) can be used for Western Blotting? It's not listed as one of the applications.

    This antibody has not yet been tested in Western blotting.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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