Anti-PKD2 抗体 (ab7281)

Reviews (3)Q&A (1)References (4)

Key features and details

  • Rabbit polyclonal to PKD2
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-PKD2 antibody
    PKD2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to PKD2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide corresponding to Human PKD2 aa 849-864.

  • 特記事項

    This antibody recognizes human protein kinase D2 (PKD2), a serine threonine protein kinase. PKD2 is widely expressed in human and murine tissues and exhibits strong homology to the serine threonine protein kinases PKD/PKCmicro and PKCnu. In contrast, it has a low degree of sequence homology to other members of the PKC family. PKD2 is activated by phorbol esters and a variety of growth factors.

     This product was previously labelled as Protein Kinase D2 

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 一次抗体 備考

    This antibody recognizes human protein kinase D2 (PKD2), a serine threonine protein kinase. PKD2 is widely expressed in human and murine tissues and exhibits strong homology to the serine threonine protein kinases PKD/PKCmicro and PKCnu. In contrast, it has a low degree of sequence homology to other members of the PKC family. PKD2 is activated by phorbol esters and a variety of growth factors.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 105 kDa.

ターゲット情報

  • 機能

    Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.

  • 組織特異性

    Widely expressed.

  • 配列類似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylation of Ser-876 correlates with the activation status of the kinase.
    Ser-706 is probably phosphorylated by PKC.

  • 細胞内局在

    Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DKFZp586E0820 antibody
      • HSPC187 antibody
      • KPCD2_HUMAN antibody
      • nPKC D2 antibody
      • nPKC-D2 antibody
      • PKD2 antibody
      • PRKD 2 antibody
      • Prkd2 antibody
      • Protein kinase D2 antibody
      • Serine/threonine protein kinase D2 antibody
      • Serine/threonine-protein kinase D2 antibody
      see all

    画像

    • Western blot - Anti-PKD2 antibody (ab7281)
      Western blot - Anti-PKD2 antibody (ab7281)Image courtesy of an anonymous Abreview.
      Anti-PKD2 antibody (ab7281) at 1/500 dilution + whole cell lysate prepared from murine NIH3T3 cells at 25 µg

      Secondary
      Donkey anti-rabbit HRP at 1/5000 dilution

      Developed using the ECL technique.

      Exposure time: 15 minutes

    参考文献 (4)

    ab7281 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7281 は 4 報の論文で使用されています。

    • Xiao Y  et al. Deficiency of PRKD2 triggers hyperinsulinemia and metabolic disorders. Nat Commun 9:2015 (2018). PubMed: 29789568
    • Arun SN  et al. Ultraviolet B irradiation and activation of protein kinase D in primary mouse epidermal keratinocytes. Oncogene : (2010). WB ; Mouse . PubMed: 21132013
    • Li J  et al. PKD1, PKD2, and Their Substrate Kidins220 Regulate Neurotensin Secretion in the BON Human Endocrine Cell Line. J Biol Chem 283:2614-21 (2008). ICC/IF ; Human . PubMed: 18048355
    • Jackson LN  et al. Overexpression of wild-type PKD2 leads to increased proliferation and invasion of BON endocrine cells. Biochem Biophys Res Commun 348:945-9 (2006). PubMed: 16899224

    レビューと Q&A

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    1-4 of 4 Abreviews or Q&A

    Question

    I am using this antibody in mouse myoblasts. The antibody gives a distinct, clear band at the approximate expected size. However, I am interested in possible cross-reactivity with closely-related PKD1. Does this antibody cross-react with PKD1? What is the sequence of the immunogen that the antibody was raised against? Thank you.

    Read More
    Answer

    Thank you for your inquiry.

    This antibody was raised against the full length human Protein Kinase D2, plus it is a polyclonal, so the antibody will have multiple binding epitopes along the protein.

    I've BLASTed the immunogen against mouse Protein Kinase D1 and it shows 69% homology. Generally we say that anything with >85% sequence homology would be expected to cross-react, so this is quite low. However, we have never tested this antibody for its cross-reactivity with Protein Kinase D1 so we are unsure if it cross-reacts.

    I hope this information helps. Please contact us with any other questions.

    Read More

    Western blot abreview for Anti-PKD2 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (NIH 3T3)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    25 µg
    Specification
    NIH 3T3
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 23 2010

    Western blot abreview for Anti-PKD2 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HMEC)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    15 µg
    Specification
    HMEC
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 22 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Protein Kinase D2 antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (placenta tissue-paraffin sections)
    Specification
    placenta tissue-paraffin sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 5%
    Read More

    Dr. Daniel Macphee

    Verified customer

    投稿 Jan 30 2006

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