Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
No
Fixative
Methanol
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/400
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit AlexaFluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
Asynchronous cells were MeOH fixed, immunofluorescently labeled and counterstained with DAPI. This antibody produces the expected cytoplasmic focal staining pattern. PF fixed samples were also evaluated and produced a focal staining pattern that was slightly enriched within nuclei.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Jan 23 2014