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    pkc-iota-phospho-t555--t563-antibody-ab5813.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC
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Anti-PKC iota (phospho T555 + T563) 抗体 (ab5813)

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Western blot - Anti-PKC iota (phospho T555 + T563) antibody (ab5813)

    Key features and details

    • Rabbit polyclonal to PKC iota (phospho T555 + T563)
    • Suitable for: Flow Cyt, WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

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    関連製品

    製品の概要

    • 製品名

      Anti-PKC iota (phospho T555 + T563) antibody
    • 製品の詳細

      Rabbit polyclonal to PKC iota (phospho T555 + T563)
    • 由来種

      Rabbit
    • 特異性

      This antibody reacts with PKC lambda immunoprecipitates, indicating cross-reactivity for PKC lambda [pT563]. PKC zeta [pT560] (83% homologous) has been shown to cross-react by peptide competition. Peptide competition also suggests that this antibody may partially cross-react with PKC beta 1 [pS642] (58% homologous) and PKC nu [pT655] (42% homologous).
    • アプリケーション

      適用あり: Flow Cyt, WBmore details
    • 種交差性

      交差種: Mouse, Human
    • 免疫原

      Synthetic peptide corresponding to PKC iota (phospho T555 + T563).

    • ポジティブ・コントロール

      • WB: Jurkat cells.
    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • バッファー

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol, 0.1% BSA
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 特記事項(精製)

      The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC iota. The final product is generated by affinity chromatography using a PKC iota-derived peptide that is phosphorylated at threonine 555.
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • PKC
      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • Regulation

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human PKC iota protein (ab60850)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab5813の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use at an assay dependent concentration.
    WB
    1/1000. Detects a band of approximately 76 kDa.
    特記事項
    Flow Cyt
    Use at an assay dependent concentration.
    WB
    1/1000. Detects a band of approximately 76 kDa.

    ターゲット情報

    • 機能

      Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. May play a role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. Functions in both pro- and anti-apoptotic pathways. Functions in the RAC1/ERK signaling required for transformed growth. Plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs).
    • 組織特異性

      Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers.
    • 配列類似性

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 OPR domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • ドメイン

      The OPR domain mediates interaction with SQSTM1.
      The C1 domain does not bind diacylglycerol (DAG).
    • 翻訳後修飾

      On neuronal growth factor (NGF) stimulation, phosphorylated by Src on Tyr-265, Tyr-280 and Tyr-334. Phosphorylation on Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation.
    • 細胞内局在

      Cytoplasm. Membrane. Endosome. Nucleus. Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by cSrc, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Vesicular tubular clusters. Transported to VTCs through interaction with RAB2A.
    • Target information above from: UniProt accession P41743 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 5584 Human
      • Entrez Gene: 18759 Mouse
      • Omim: 600539 Human
      • SwissProt: P41743 Human
      • SwissProt: Q62074 Mouse
      • Unigene: 478199 Human
      • Unigene: 291554 Mouse
      • 別名

        • aPKC lambda/iota antibody
        • aPKC-lambda/iota antibody
        • Atypical protein kinase C lambda/iota antibody
        • Atypical protein kinase C-lambda/iota antibody
        • DXS1179E antibody
        • KPCI_HUMAN antibody
        • MGC26534 antibody
        • nPKC iota antibody
        • nPKC-iota antibody
        • nPKCiota antibody
        • PKC lambda antibody
        • PKC lambda/iota antibody
        • PKCI antibody
        • PKCiota antibody
        • PKClambda antibody
        • PRKC iota antibody
        • PRKC lambda/iota antibody
        • PRKC-lambda/iota antibody
        • PRKCI antibody
        • Protein kinase C iota antibody
        • Protein kinase C iota type antibody
        see all

      画像

      • Western blot - Anti-PKC iota (phospho T555 + T563) antibody (ab5813)
        Western blot - Anti-PKC iota (phospho T555 + T563) antibody (ab5813)
        Peptide Competition and Phosphatase Treatment: Lysates prepared from Jurkat cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda  phosphatase (5), blocked with a 5% low-fat milk-TBST buffer for one hour at room temperature, and incubated with ab5813 antibody for two hours at room temperature in a 3% low-fat milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate  and bands were detected using the Pierce SuperSignalTM method. The data show that the phosphopeptide corresponding to PKC iota [pT555] blocks the antibody signal. The peptide corresponding to PKC zeta [pT560] blocks the antibody signal and the peptides corresponding to PKC isoforms beta 1 [pT642] and gamma [pÔ655] partially block the antibody signal (data not shown), suggesting cross-reactivity of the antibody with these sites. The antibody signal was not blocked by the corresponding peptides of any other PKC isoforms. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

        Peptide Competition and Phosphatase Treatment: Lysates prepared from Jurkat cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% low-fat milk-TBST buffer for one hour at room temperature, and incubated with ab5813 antibody for two hours at room temperature in a 3% low-fat milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the phosphopeptide corresponding to PKC iota [pT555] blocks the antibody signal. The peptide corresponding to PKC zeta [pT560] blocks the antibody signal and the peptides corresponding to PKC isoforms beta 1 [pT642] and gamma [pÔ655] partially block the antibody signal (data not shown), suggesting cross-reactivity of the antibody with these sites. The antibody signal was not blocked by the corresponding peptides of any other PKC isoforms. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

      プロトコール

      • Western blot protocols

      Click here to view the general protocols

      データシートおよび資料

      • Datasheet download

        Download

      参考文献 (20)

      ab5813 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab5813 は 20 報の論文で使用されています。

      • Dey A  et al. The interruption of atypical PKC signaling and Temozolomide combination therapy against glioblastoma. Cell Signal 77:109819 (2021). PubMed: 33147518
      • Patel R  et al. Simultaneous inhibition of atypical protein kinase-C and mTOR impedes bladder cancer cell progression. Int J Oncol 56:1373-1386 (2020). PubMed: 32236625
      • Kotula-Balak M  et al. Towards understanding leydigioma: do G protein-coupled estrogen receptor and peroxisome proliferator-activated receptor regulate lipid metabolism and steroidogenesis in Leydig cell tumors? Protoplasma 257:1149-1163 (2020). PubMed: 32180008
      • Ito M  et al. Targeting PKC?-PAK1 in EGFR-mutation positive non-small cell lung cancer. Transl Lung Cancer Res 8:667-673 (2019). PubMed: 31737502
      • Li HS  et al. Protein Kinase C Lambda Mediates Acid-Sensing Ion Channel 1a-Dependent Cortical Synaptic Plasticity and Pain Hypersensitivity. J Neurosci 39:5773-5793 (2019). PubMed: 31101759
      View all Publications for this product

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      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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